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The design of a 50 Hz single longitudinal mode, diode-pumped and frequency-tripled Nd:YAG master oscillator power amplifier is described, and the first measurements of output parameters are presented. The laser oscillator is injection-seeded by a tuneable monolithic Nd:YAG ring laser and frequency stabilized by minimising the Q-switch build-up time. The laser system will be an integral part of an airborne instrument demonstrator for a first satellite based Doppler wind lidar to measure vertical profiles of one component of the atmospheric wind vector. This paper focuses on the investigation of the frequency jitter and the linewidth of the laser, which are measured on a pulse-to-pulse basis. For this purpose a compact, high accuracy beat frequency monitoring system has been developed at DLR. By operating the amplifier stage at half the repetition rate (50 Hz) of the oscillator, we could reduce the frequency stability from 10 MHz (rms) to 1.3 MHz (rms) (over a 14 s period). We have determined a mean linewidth of 15 MHz (FWHM) at 1064 nm. These measured laser parameters enable wind velocity measurements in the atmosphere (0-15 km) at an accuracy of 1 to 2 m/s.

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Chromophore-assisted laser inactivation (CALI) is a light-mediated technique used to selectively inactivate proteins of interest to elucidate their biological function. CALI has potential applications to a wide array of biological questions, and its efficiency allows for high-throughput application. A solid understanding of its underlying photochemical mechanism is still missing. In this study, we address the CALI mechanism using a simplified model system consisting of the enzyme beta-galactosidase as target protein and the common dye fluorescein. We demonstrate that protein photoinactivation is independent from dye photobleaching and provide evidence that the first singlet state of the chromophore is the relevant transient state for the initiation of CALI. Furthermore, the inactivation process was shown to be dependent on oxygen and likely to be based on photooxidation of the target protein via singlet oxygen. The simple model system used in this study may be further applied to identify and optimize other CALI chromophores.