No abstract

No abstract

We investigated the localization of amylase in normal human lungs and the female genital tract using immunohistochemical and histochemical methods. Immunohistochemical procedures were applied to formaldehyde-fixed, paraffin-embedded specimens as well as to cryostat sections of periodate/lysine/paraformaldehyde (PLP)-fixed tissues. The starch-substrate-film method was used for the histochemical investigation of unfixed frozen sections. Amylase immunoreactivity was observed in ciliated epithelial cells of the bronchus and in serous cells of the bronchial glands but not in the alveolar epithelium. Immunoreactive amylase was also found in the cytoplasm of the ciliated epithelium of the fallopian tubes, especially in the apical part of the cytoplasm and in ciliary vesicles. Immunoreactive amylase was also found to be present in the surface epithelial cells and glands of the uterine cervix, as well as in the superficial part of the endometrial glands. The distribution of amylase activity revealed using histochemistry was similar to that observed in cryostat sections of PLP-fixed tissues after immunohistochemical staining. Amylase antigenicity was better preserved in cryostat sections of PLP-fixed materials than in formaldehyde-fixed, paraffin-embedded specimens. The results are discussed in relation to pulmonary and female-genital-tract diseases.

The transport problems involved in the thermal growth of thick oxide films are reexamined on the basis of a semiconductor model of the oxide film, in which the electron and hole concentrations are assumed to be in local thermodynamic equilibrium. The coupled transports of ions and of electrons or holes required in the Wagner electrochemical model of film growth are related separately to the electrostatic and Fermi potential gradients in the oxide film. The electrostatic potential difference across the oxide film is shown to be fixed by the free energy of formation of the oxide and by the transport coefficients of the several migrating species. The effect of externally applied fields on the thermal growth process is discussed. Anomalies pointed out by Raleigh are shown not to be inconsistent with the electrochemical model of film growth. The question of local electroneutrality in the film relative to the parabolic growth law is also examined.

No abstract