In medical analysis laboratories, techniques for identifying bacteria are currently becoming more and more numerous. The objective of this study is to compare the 2 bacterial identification systems Bis NEG-D and Api 20E for the identification of gram-negative bacilli. This is a qualitative evaluation of the Bis NEG-D compared to the gold standard Api 20E. During the study period, 32 Gram-negative bacilli isolates were identified simultaneously using Api 20E and Bis NEG-D. The samples are represented by 12 (37.5%) blood samples for blood culture, 12 (37.5%) urine samples for cytobacteriological examination of the urine, 06 (18.8%) pus samples for bacteriological examination of pus, a sample of the cerebrospinal fluid (3.1%) and a vaginal sample (3.1%).The bacteria identified were represented by Enterobacter spp, Escherichia col,i Klebsiella pneumonia, Shigella spp, Salmonella typhi, Acinetobacter baumannii, Pseudomonas spp, Proteus mirabilis, Raoultella ornithinolytica and Bukholderia cepacia. This study showed a concordance of 90.6% (29/32) and a discordance of 9.4% (3/32) between the results of Api 20E and Bis NEG-D. Concerning the probability scores, they vary between 95% to 100% for Api 20E and between 79.3% to 100% for Bis NEG-D. This study also compared the pros and cons of using Api 20 E and Bis NEG-D. The Bis-NEG-D is valid and can be used by medical analysis laboratories like the Api 20E, especially if these laboratories do not need to perform a lot of bacterial identification tests.

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In whole, 56 patients were included. Amidst identified microorganisms were fungus (4,7%) and bacteria (95,3%) to which Gram negative bacilli represented 72,1% (n=44), Gram positive cocci 6,4% (n=10), Gram positive bacilli 8,2% (n=5) and Gram negative cocci 3,3% (n=2). Among these bacterias, Pseudomonas aeruginosa and Proteus sp were predominant, with respectively 41% (n=25), 23% (n=14). However, three cases of S. aureus reported, six with negative coagulase Staphylococcus, one with Escherichia coli, one with Klebsiella sp, one with Haemophilus sp, two cases with Neisseria sp and four cases with Corynebacterium sp. Two types of cultures were noticed, one of them monomorphic (91,1%, n=51) and the other polymorphic (8,9%, n=5) to which 3 associations of P. aeruginosa-Proteus sp, 1 association of P. aeruginosa- coagulase negative Staphylococcus and 1 association of P. aeruginosa- E. coli. No resistance to ciprofloxacin was observed with Pseudomonas, Neisseria sp, Haemophilus, and enterobacteria except for E. coli. No resistance to rifampicin was observed with S. aureus. However, the sensitivity of S. aureus to ciprofloxacin decreased (one bacterium out of three). The use of rifampicin or fluoroquinolones should be based on the type of ear infections,