Background Lipid and inflammatory molecules play a key role in the development of inflammation. Neutrophil counts are used as markers of inflammation duration, and HDL-C is used as an anti-atherosclerosis component. However, few studies have been found to integrate these two indicators to explore coronary stenosis. We suggested that neutrophil count as a marker of inflammation persistence and HDL-C as an anti-atherosclerotic component should be integrated into a single biomarker NHR to explore its correlation with CAD degree and predict the severity of coronary stenosis among CAD patients. Methods We examined 404 eligible patients who underwent coronary angiography. Based on the results of coronary angiography, patients in CAD+ group (n = 155) were defined as those having angiographic coronary stenosis of at least 50% lumen reduction in at least one major coronary artery (including left anterior descending artery, left circumflex artery, left main coronary artery, right coronary artery). Patients with luminal stenosis but no more than 50% were defined as CAD− group (n = 49), and patients without luminal stenosis (n = 200) were regarded as control group. The relationship between various serum markers and the severity of coronary stenosis was examined by Spearman correlation analysis. Logistic regression analysis was performed to identify the influencing factors of the severity of coronary artery disease. Results The modified Gensini score was positively correlated with neutrophil HDL-C ratio and negatively correlated with albumin and HDL-C. Multiple regression analysis showed that neutrophil HDL-C ratio were significantly associated with CAD. Neutrophil HDL-C ratio is an independent predictor of CAD. The ROC analysis provided a cut-off value of 1.51 for neutrophil HDL-C ratio to predict CAD with 94.8% sensitivity and 0.024 Yoden index, and area under the ROC curve of 0.617 (95% CI 0.560–0.675, P < 0.001). Conclusion Neutrophil HDL-C ratio is not only closely related to coronary artery stenosis, but also an independent predictor of severe coronary stenosis.

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Background: To evaluate the effect of berberine (BBR) intervention on left ventricular hypertrophy and systolic function in rats by ultrasound layered strain imaging and cardiac hypertrophy model.Methods: Eighty healthy male Sprague-Dawley (SD) rats were randomly divided into four groups: group A (normal saline control group), group B [isoproterenol (ISO) induced model group], group C (BBR hydrochloride 5 mg/kg + ISO group) and group D (BBR hydrochloride 10 mg/kg + ISO group).Echocardiography was performed on days 1, 7 and 14, respectively. The myocardial tissue was taken for pathological examination. The key proteins of Rho/ROCK signaling pathway were quantified by immunohistochemical staining.Results: On day 7, compared with group A, peripheral strain values of the subendocardium and middle myocardium of rats in groups B, C and D were significantly decreased. The absolute value of circumferential strain (CS) in subendocardium and middle myocardium in group B was significantly lower than that in

Background: Lipid and inflammatory molecules play a key role in the development of coronary atherosclerosis. Hypersensitive C-reactive proteins are used as markers of inflammation duration, and HDL-C is used as an anti-atherosclerosis component. However, few studies have combined the two indicators to explore coronary stenosis. We suggested that Hypersensitive C-reactive proteins as a marker of inflammation persistence and HDL-C as an anti-atherosclerosis component should be integrated into a single biomarker , so as to explore the correlation of Hypersensitive C-reactive protein HDL-C ratio with the severity of coronary stenosis and to predict the severity of coronary stenosis in CAD patients.Methods: We examined 360 eligible patients who underwent coronary angiography. Based on the results of coronary angiography, patients with any major coronary arteries (the left anterior descending artery, the left circumflex artery, the left main coronary artery, the right coronary artery) whose lumen diameter reduced by more than 50% were defined as CAD + group (n = 139) .Patients with luminal stenosis but no more than 50% were defined as CAD- group (n = 41), and patients without luminal stenosis (n = 180) were regarded as control group. The relationship between various serum markers and the severity of coronary stenosis was examined by Spearman correlation analysis. Logistic regression analysis was performed to identify the influencing factors of the severity of coronary artery disease.Results: The modified Gensini score was positively correlated with Hypersensitive C-reactive protein HDL-C ratio . Multiple regression analysis showed that Hypersensitive C-reactive protein HDL-C ratio were significantly associated with CAD. Hypersensitive C-reactive protein HDL-C ratio is an independent predictor of CAD. The ROC analysis provided a cut-off value of 1.17 for Hypersensitive C-reactive protein HDL-C ratio to predict CAD with 83.9% specificity and 0.242 Yoden index, and area under the ROC curve of 0.632 (95%CI 0.571-0.694, P <0.001).At the same time, the area under the ROC curve of Neutrophil HDL-C ratio was 0.620, indicating that Hypersensitive C-reactive protein HDL-C ratio as a predictor of CAD has better diagnostic performance than Neutrophil HDL-C ratio.Conclusion: Hypersensitive C-reactive protein HDL-C ratio is not only closely related to coronary artery stenosis, but also an independent predictor of severe coronary stenosis.

Background We aimed to detect the cardiac dysfunction in the early myocardial hypertrophy rat model by layered strain and to evaluate the effect of berberine on the left ventricular systolic function of myocardial hypertrophy rats. Methods 80 healthy SD rats were randomly divided into 4 groups. The last three groups(ISO group) were injected with isoproterenol intraperitoneally to establish a model of myocardial hypertrophy;After successful modeling, group A and group B were given berberine hydrochloride intragastric administration (5mg/kg; 10mg/kg), and group C was given the same amount of normal saline by intragastric administration. The left ventricular systolic function was performed at the end of each trial using the layered strain technique. The myocardial tissue of rats was taken for pathological examination, and Masson trichrome staining and hematoxylin-eosin staining were performed. The myocardial interstitial fibrosis was evaluated and the correlation analysis was performed. Results The GCS, GSCendo and GSCmid of the ISO group were lower than those of the control group, and the difference was statistically significant (− 20.46% −29.57% −19.39% vs. −15.71% −20.11% −15.35%; P < 0.05). After berberine treatment, the GSCendo in group A (5mg/kg) and group B (10mg/kg) was higher than that in group C (− 24.49% vs.−26.87% vs.−20.09%; P < 0.001); The improvement in group B was more significant (− 24.49% vs.−26.87%; P < 0.001). Pathological results showed that, compared with the control group, fibrosis was significantly increased in the ISO group. After 7 days of berberine intragastric intervention, compared with group C without intervention, the fibrosis deposition in group A and B was reduced, and the fibrosis deposition in group B was more obvious. Spearman rank correlation analysis showed that Gscendo was positively correlated with the percentage of myocardial interstitial fiber deposition. Conclusion Persistent cardiac hypertrophy leads to marked myocardial fibrosis and necrosis. Berberine can inhibit the progression of myocardial fibrosis after the formation of cardiac hypertrophy. The stratified strain technique can identify early myocardial systolic dysfunction in patients with cardiac hypertrophy and evaluate the efficacy of short-term drug intervention, which is expected to be a new method for clinical quantitative evaluation of left ventricular systolic dysfunction.