Background: Molar pregnancy (hydatidiform mole HM) is an abnormal form of pregnancy in which a non-viable fertilized egg implants in the uterus and will fail to come to term. A molar pregnancy is a gestational trophoblastic disease which grows into a mass in the uterus that has swollen chorionic villi. These villi grow in clusters that resemble grapes. A molar pregnancy can develop when a fertilized egg does not contain an original maternal nucleus. The diagnosis of HM is based on its morphology. In classic cases, the abnormally large villi have a vesicular or a grape like appearance. Histologically, HM presents variable degrees of circumferential trophoblastic hyperplasia and epithelial atypia. Distinguish between complete mole (CM) and partial mole (PM) is difficult especially with early diagnosis and evacuation of molar pregnancies, however, the criteria are subjective and show considerable interobserver variability. Unfortunately in our country the histological diagnosis is depend only on routine stain (H&E) which is not giving a clear results in some cases because of that, the study design to find the similarities and differences between the two types of molar pregnancy by using some of special stains which surely distinguish between complete and partial moles samples using immunohistochemical marker P57KIP2. Objective: To detecting if the special stain can be helpful to distinguish between the two types of molar pregnancy also to find the accuracy of the routine diagnosis of hydatidiform mole comparing with P57 immunohistochemistry stain. Patients and Methods: The study conducted at the maternal teaching hospital in Erbil city. Thirty cases of Molar Pregnancy diagnosed, 15 of them were complete and the others were partial mole. All the samples studied histologically using H&E and some of special stains including: PAS stain, Congo red, and Trichrom, to describe the morphological differences between the two types of mole. Also immunohistological P57 stain was used in this study to confirm the type of mole. Results: The study result shows the morphological variation between complete and partial hydatidiform through using the routine H&E stain and special stains. The complete hydatidiform mole sections showed vesicular swelling of chorionic villi and circumferential trophoblastic hyperplasia, the trophoblastic proliferation grade varying in complete samples, most samples showed grade II (46.6%) of trophoblastic proliferation, while few of samples showed grade III (13.3%). The cases of partial hydatidiform mole showed scattered hydropic villi with irregular scalloped villous outlines and mild trophoblastic hyperplasia, trophoblastic

Background: Isotretinoin is a vitamin derivative also known as 13-cis-retinoic acid used in the treatment of severe acne and some forms of skin, head and neck cancer. Some studies have shown that isotretinoin induces apoptosis in various cells in the body. Isotretinion has not been clearly implicated in cases of clinically apparent acute liver injury with jaundice. The mechanism by which isotretinoin causes serum aminotransferase elevations is not known, but it may represent a direct toxic effect, in that it appears to be more frequent with higher dose therapy. For prevention or healing natural herb and its oil are used. The Cumin oil is one of these herbal oils which used as antioxidant and anti-inflammatory. Objective: To evaluate the histological damage of liver after treatment with the Isotretinoin and find out if cumin oil which was anti-inflammatory natural herb can protect liver from damage. Patients and Methods: 30 male wistar rats weighing 200-250g were used in this study divided into five groups: the first group was control, second was treatment group (T1), receive 10 mg of Isotretinion; the third group was (T2) receives 20 mg of Isotretinion. And last two others were cumin oil groups including (T3) receive 10 mg of Isotretinion plus Cumin oil and (T4) receive 20 mg of Isotretinion plus Cumin oil. The experiment was accomplished in three weeks. In the end of each week, animals from each group were killed and dissect and liver taken for histopathological study. Blood collected for liver functional enzymes evaluation. Liver functional enzymes include alkaline phosphatase, S -GOT, S-GPT, T.S.B, Bilirubin direct and Bilirubin indirect. Results:The study results show a significant difference between the control groups and treatment (T1) group in the values of alkaline phosphatase. A significant difference also found in the result of serum GOT between the T2, T3and T4 groups. The T3 group shows the highest significance between the other groups. The result of GPT shows no significant differences between the study groups except T3 group which show significant P˂0.05. The data of total serum bile (T.S.B) and indirect bilirubin test shows no significant differences among the study groups while analyzing data of direct bilirubin shows significant differences in T1and T2 comparing to other study groups graph. The histological examination of the rat's liver shows variable changes in the treatment groups during different time of the study. Isotretinion affect the texture of liver tissue mains noticed alteration includes infiltration of inflammatory cell around central vein, congestion of sinusoid and vacuolar degeneration in

Introduction Background and objective: Infertility and poor ovarian response are serious problems in our society; fortunately the in vitro fertilization and intracytoplasmic sperm injection technique dissolve many of these problems. This study aimed to assess the effect of an individualized GnRH antagonist regimen on intracytoplasmic sperm injection process especially the aptness of it in given mature reiterative oocytes, normal fertilization, and acceptably zygote cleavage which enhance pregnancy. Methods: The population of the study consisted of 877 couples attending the in vitro fertilization-infertility center in the maternity teaching hospital and chose to undergo intracytoplasmic sperm injection after using of antagonist protocol for stimulation of women ovaries. The women ages ranged between 24 and 44 years. The collected data included a number of reiterative oocytes; fertilization, embryo grades and lastly estimates the pregnancy results Results: A significant association (P <0.001) was found in the numbers of reiterative oocytes between the age groups of the study. A significant association (P = 0.005) was also found concerning to quality of fertilization process. The study of the zygote cleavage and blastomeres formation followed the fertilization show variable grade of embryos and analyzed data of embryo grades in this study indicate presence of significant association (P = 0.003). The rate of pregnancy showed significant association between the groups of the study with the <30 years age group have chance of pregnancy higher than other groups. Conclusion: The antagonist protocol of ovary stimulation according to the results of this study is a qualified protocol; besides it is rapid and can be reversed. This protocol can give effective results in the intracytoplasmic sperm injection process especially for young women, and enhance the pregnancy.