No abstract

Possibilities and prospects of correcting women's health disorders associated with hypoestrogenic conditions of various etiologies or hormonal imbalances are considered. The causes of estrogen deficiency and features of complaints in women of different ages with an emphasis on peri- and menopause are systematized. An alternative to menopausal hormone therapy (MHT) with the use of phytovitamin preparations is considered. The relationship of hormonal metabolism with vitamin and mineral status, feasibility and benefits of complex phyto-, vitamin- and mineral preparations is shown. The therapeutic efficacy of medicinal plants that are part of the complex phytopreparation "Forevia Maxi" ("Organosyn LTD") in accordance with the symptoms of premenstrual syndrome (PMS): vasomotor (shortness of breath, hot flushes, night sweats, blood pressure fluctuations), genitals and atrophy of the vagina, itching, burning, cystalgia, urinary incontinence, dyspareunia) complications, memory and cognitive impairment, decreased sexuality, libido, sleep disorders, mood swings, anxiety, depression, aggravating psycho-emotional state, osteoporotic changes. Data from clinical trials of phytoextracts are shown, which demonstrate that the effect of the components of Forevia Maxi significantly exceeds the effect of placebo, and sometimes not less than with the use of MHT. The obvious benefits of this tool and as an adjunct to the basic therapy of PMS and hypoestrogenic conditions, characterized by ease of use, good safety profile, allows you to individualize the approach to patients, which is important in cases of medical contraindications for hormone therapy. Forevia Maxi is also the most appropriate for use with MHT, as an additional component of the correction of hypothalamic-pituitary disorders and saturation of the body with essential minerals and vitamins.

* Роботу виконано згідно з плановою науковою тематикою Національного фармацевтичного університету «Фармакологічне дослідження лікарських засобів, що впливають на функцію репродуктивної системи. Фармакологія простатопротекторів, токолітиків, гравідопротекторів, топікальних лікарських форм для лікування вагінітів, коректорів еректильної дисфункції», (державний реєстраційний № 0103U000478). Установою, що фінансує дослідження, є МОЗ України. Автори гарантують колективну відповідальність за все, що опубліковано в статті. Автори гарантують відсутність конфлікту інтересів та фінансової зацікавленості при виконанні роботи та написанні статті. Рукопис надійшов до редакції 12.01.2019.

Aim. The research of antimicrobial and toxicological parameters of a promising pharmaceutical composition with indole-3-carbinol and meloxicam in the form of rectal suppositories. Materials and methods. The research of antimicrobial activity was carried out in vitro by diffusion in nutrient agar in the modification of "holes" on the reference strains of common pathogens Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Candida albicans. Adult rats were used for the study of acute toxicity. Suppository mass were administrated in the largest possible volume rectally or orally. The animals were periodically monitored according to the experimental plan – the assessment of physiological parameters before administration and after 6, 12, 18, 24 hours, at 3, 7 and 14 days. The animals were removed from experiment and necropsy provided after 1, 3, 7, and 14 days. Results. The sizes of zones of inhibition of the microorganisms growth were most significant (from 19.27±0.61 mm of E. coli to 40.80±0.42 mm of S. aureus) near sample of the combined composition suppository compared with other combination of active substances and excipients. During the observation of animals for 14 days and the study of internal organs after autopsy, deviations in physiological (weight, temperature, activity, respiratory rate) and macroscopic morphological indicators of animals from reference values were not detected. Conclusion. According to the results of microbiological studies, a moderate antimicrobial effect of suppositories of combined composition in relation to all the studied pathogens was revealed. The absence of manifestations of acute toxicity allows us to conclude that the pharmaceutical composition can be classified as practically non-toxic substances. The obtained results allow us to recommend a pharmaceutical composition with indole-3-carbinol and meloxicam on a polyethylene oxide basis in the form of suppositories for further preclinical studies of specific pharmacological effects as a prostate protective agent.

The review is dedicated to the modern ideas about the composition of the human intestinal microbiome, factors that determine the bacterial «landscape» and affect its activity. The main functions of normal microflora have been described, including intestinal motility, protection of intestinal barrier against pathogenic bacteria, parasites, intestinal epithelial regeneration, metabolic and immunological functions, participation in digestive processes, synthesis of amino acids and proteins, antibiotics, vitamins, hormonally active substances, promoting the absorption of minerals and nutrients, preventing the development of pathological conditions. Determination of intestinal microbiota expression of the innate immune system explains the role of microflora in chronic inflammation and diseases such as liver fibrosis, metabolic syndrome, type 2 diabetes mellitus, atherosclerosis, cardiovascular, neurodegeneration and cancer. A promising way is the use of microorganisms with beneficial properties (probiotics), the necessary substrate for them (prebiotics), their metabolites (metabiotics) and complexes of pro‑ and prebiotics (synbiotics) not only to restore and regulate the intestinal microflora, but also as therapeutic agents in some diseases, in particular during eradication therapy of the bacterium Helicobacter pylori. The functions of some species and strains of beneficial bacteria have being discussed, in particular the strain Bacillus causii UBBC‑07, as well as the results of preclinical and clinical trials of its use at antibiotic‑associated diarrhea in the inhibition of Clostridium difficile. The issues of safety and facts of positive impact on human health of probiotic products of Probeez line (Organosyn Ltd.) are considered, which differ in the personification of appointments to certain segments of the population: Probeez®, Probeez® Femina, Probeez® Kids, Probeez® DUO and Probeez® Immuno contain successfully selected live probiotic bacteria. The available range of monocomponent (Probeez® with Kids, Probeez® DUO), multicomponent (Probeez®, Probeez® Femina) and synbiotic (Probeez® Immuno) products allows to choose the best option in specific conditions for their application not only in inflammatory diseases of the gastrointestinal tract, but also in chronic disorders of other systems and organs.