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An experimental platform has been developed for rapid sampling and quenching of chemostat cultivated Penicillium chrysogenum broth for metabolome analysis in highly dynamic experiments, aimed at the elucidation of the in vivo kinetic properties of metabolism. The sampling and quenching protocol available from Saccharomyces cerevisiae had to be modified for Penicillium chrysogenum mainly because of its filamentous character. Intracellular metabolites of glycolysis, TCA cycle, and adenine nucleotides were measured with isotope dilution mass spectrometry (IDMS) using a U-(13)C-labeled metabolite mix produced from yeast cells as internal standard. By addition of the U-(13)C internal standard mix prior to the metabolite extraction procedure, partial degradation of metabolites as well as non-linearity and drift of the LC-MS/MS could be successfully compensated for. It was found that there is a serious matrix effect on metabolite extraction between different organisms, which is however completely corrected for by the IDMS approach. Intracellular metabolites could be analyzed with standard deviations of around 5%. A comparison of the metabolite levels between Saccharomyces cerevisiae and Penicillium chrysogenum showed both significant similarities and large differences, which seem to be related to the presence of the penicillin pathway.

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The purpose of this study was to determine the growth response of meat chickens due to the addition of probiotics and digestive enzymes in the fermented diet containing maggot flour and local materials. The research was carried out at Field Laboratory of Animal Science, University of Syiah Kuala-Banda Aceh, Indonesia for 66 days. A total of 100 meat chickens were designed using a completely randomized design (CRD) consisting of 5 treatments, namely P0 = 100% fermented ration (control), P1 = 0.5% probiotic + 0% digestive enzymes + 100% fermented diet, P2 = 0% probiotic + 0.5% digestive enzymes + 100% fermented diet, P3 = 0.5% probiotic + 1% digestive enzymes + 100% fermented diet, P4 = 1% probiotic + 0.5% digestive enzymes + 100% fermented diet with 4 replications. The data obtained were analyzed using ANOVA (Analysis of variance) and significant difference of data was analyzed by Duncan’s multiple distance test. The results showed that the addition of probiotics and digestive enzymes with different levels of administration in the fermented diet had a significant effect (P<0.05) on the increase in final body weight and percentage of protein retention. The best results were obtained on chicken fed on fermented diet contained 0, 5% digestive enzymes by increasing on final body weight, protein retention, feed conversion and feed efficiency which were higher than fermented feed containing probiotics. In conclusion, it was well known that digestive enzyme effected synergistically on fermented diet in increasing protein retention resulted a higher final body weight of meat chicken.