Human immunodeficiency virus (HIV) is associated with altered metabolism and increased energy expenditure, this energy requirement increases significantly as the HIV disease progresses. This study aimed on the use of Adenosine triphosphate (ATP), Guanosine triphosphate (GTP), Adenosine diphosphate (ADP) and Adenosine monophosphate (AMP) as an index of energy utilization, storage and energy balance in HIV infected individuals. This is a longitudinal, prospective, case-controlled study involving seventy seven (77) HIV Sero-positive individuals newly diagnosed attending retroviral disease treatment centre of Nnamdi Azikiwe University University Teaching Hospital (NAUTH) aged 18-60 years both male and female not on highly active antiretroviral therapy (HAART), were enrolled in the study as test subjects and thirty six (36) apparently healthy HIV Sero-negative individuals both male and female as control subjects. ATP, GTP, ADP and AMP were estimated by enzyme linked immunosorbent assay (ELISA), while, total Energy Balance was determined by calculation. The data obtained were subjected to statistical analysis using SPSS software application (version 21.0) and the results expressed as mean ± standard deviation. The plasma ATP and GTP were significantly lower (P<0.05) in both HIV pre-treatment and post-treatment group compared with control group. Meanwhile, the plasma level of ADP and AMP were significantly lower (P<0.05) in HIV post-treatment group compared   with HIV pre-treatment and control group. There was also a significant difference (P<0.05) in ATP, ADP, AMP and GTP level between HIV pre-treatment and post-treatment group. Meanwhile, the energy balance was lower (P<0.05) in HIV groups compared with control group. However, the energy balance in HIV post-treatment group was significantly lower (P<0.05) compared to HIV pre- treatment group. In conclusion, the significant changes in the biochemical parameters measured suggest altered metabolism, increased energy expenditure and energy deficit/negative energy balance in HIV subjects resulting from increased energy expenditure. Hence, High energy molecules such ATP, ADP, GTP and AMP can be used to predict early energy deficit and manage energy imbalance in HIV infected individuals.

The investigation of urinary tract infection (UTI) among diabetic patients 15-51 years and above was assessed using 100 mid-stream urine specimen with the objective of isolating and identifying different types of bacteria and their respective frequencies among diabetic patients attending diabetic clinic at Alex Ekwueme Federal University Teaching Hospital, Abakaliki. A urine culture was performed combined with a full report of urine to establish the diagnosis. The result showed that the majority of bacteria in urinary tract infections were in 27-32 years of age group (71.4%) and lowest in 15-20 years age group (0%). The predominant bacteria isolates and their percentage occurrences include; Escherichia coli (39.13%), Klebsiella pneumonia (21.74%), Proteus (8.69%), Pseudomonas aeruginosa (8.69%), Streptococcus (8.69%), Staphylococcus aureus (6.52%), Enterococcus faecalis (4.25%). There was a high prevalence of the isolated organisms in female (47.7%) compared to males (36%). It follows that most predominant agent of UTI in diabetic patients in Abakaliki Metropolis is Escherichia coli followed by Klebsiella pneumonia.

This was a cross-sectional study aimed to evaluate the use of albumin, hepatic lipase (HL) and lipoprotein lipase (LPL) enzyme as predictive markers of treatment failure in HIV-1 infected individuals. 154 participants {40 (group A), 35 (group B) on antiretroviral drugs (Test group) and 79 (group C) HIV naive participants (Control group)} aged 18 and 65 years were randomly recruited. Blood sample was collected from each test participant 6 months apart and once from control for determination of Albumin, HL, LPL, viral load (VL), CD4+  cells count. VL was significantly decreased while, Albumin, HL and LPL activities were significantly higher in test participants when compared with control P ≤ 0.05 respectively). Biochemical markers in test participants at 6 months of therapy were significantly lower compared with 12 months of therapy (P ≤ 0.05). Albumin and VL correlated positively with CD4  count while,  lamivudine, nevirapine, tenofovir, HL, LPL correlated strongly and negatively with VL (P < 0.05 respectively). The high sensitivities and positive predictive value of albumin showed their predictive superiority over CD4+ count, HL, LPL and antiretroviral drug concentrations.The study thus, concludes that hypoalbuminemia with decreased HL and LPL activities were associated with unsuppressed viral load above 1000 copies/ml. This suggests that albumin; HL and LPL are good biochemical markers for prediction of treatment failure or success in participants on antiretroviral drugs.

Diabetes mellitus is associated with neutered metabolism and higher Energy Expenditure. This study aimed on the use of Adenosine diphosphate (ADP), Flavin adenine dinucleotide (FAD), AcetylCo-enzyme A (ACA) and Nicotinamide Adenine Dinucleotide (NADH) as an index of energy utilization, storage and energy balance in Diabetic individuals. This is a longitudinal, prospective, case-controlled study involving seventy seven (77) diabetic individuals newly diagnosed attending diabetic clinic of Nnamdi Azikiwe University University Teaching Hospital (NAUTH) aged 18-60 years both male and female not on anti-diabetic drug, were enrolled in the study as test subjects and thirty six (36) apparently healthy non-diabetic individuals both male and female as control subjects. ADP, FAD, ACA and NADH were estimated by enzyme linked immunosorbent assay (ELISA), while, energy balance from macromolecules was determined by calculation. The data obtained were subjected to statistical analysis using SPSS software application (version 21.0) and the results expressed as mean ± standard deviation. The Plasma Adenosine diphosphate (ADP), Flavin adenine dinucleotide (FAD), AcetylCo-enzymeA (ACA) and Nicotinamide Adenine Dinucleotide (NADH), were significantly lower (P<0.05) in both Diabetic pre-treatment and diabetic post-treatment group compared with control groups. Furthermore, the plasma level of ACA and NADH were significantly lower (P<0.05) in DM pre-treatment group compared with DM post-treatment group. While, the plasma concentration of ADP was significantly lower in DM post-treatment groups compared with DM pre-treatment groups. However, the Calculated energy from Macromolecules was lower (P<0.05) in DM groups compared with control group. Meanwhile, the calculated energy from Macromolecules in DM pre-treatment was significantly lower (P<0.05) compared with DM post-treatment. In conclusion, the significant changes in the biochemical parameters measured suggest altered metabolism, increased energy expenditure and energy deficit/energy imbalance in diabetic subjects resulting from increased energy expenditure. Hence, energy from macromolecules such as ADP, FAD, ACA and NADH can be used to predict early energy deficit and manage energy imbalance in diabetic individuals.