This research aimed to find out the interaction effect between mixed Vesicular Arbuscular Mycorrhiza (VAM) and phosphate fertilizer to the growth of red chili (C. annuum) in acid soil, and to fnd out the best combination of mixed VAM and phosphate fertilizer to the growth of red chili (C. annuum) in acid soil. This research used an experimental method with Completely Randomized Design (CRD) in a factorial pattern with two factors. The first factor was mixed VAM dosages consisted of four levels: 0; 10; 15; 20 g/plant which contains 10 7 spores/g. The second factor was phosphate fertilizer dosages consisted of four levels: 0; 0,2; 0,4; 0,6 g/plant. Each combination treatment had three replication. The parameters were observed in the form of plant height, stem diameter, plant top dry weight, degree of VAM infection, and P content of plant tissue. Data obtained from the observation was analyzed with Analysis of Variance (ANOVA) at an error rate of 5% and 1%, treatment that showed significant or very significant result, then followed with Honestly Significant Difference (HSD) test. The result showed that interaction between mixed Vesicular Arbuscular Mycorrhiza (VAM) and phosphate fertilizer did not increase the plant height, stem diameter, and plant top dry weight, but each factor increased the plant height, stem diameter, and plant top dry weight. VAM dosage inoculation of 20 g/plant without phosphate fertilizer is the most effective combination in increasing the degree of VAM infection. Keywords:Red chili (Capsicum annuum L.), acid soil, Vesicular Arbuscular Mycorrhiza (VAM), phosphate fertilizer AbrtrakPenelitian ini bertujuan untuk mengetahui pengaruh interaksi antara Mikoriza Vesikula Arbuskula (MVA) campuran dan pupuk fosfat terhadap pertumbuhan tanaman cabai merah (C. annuum) di tanah masam serta mengetahui kombinasi dosis MVA campuran dan pupuk fosfat yang terbaik untuk pertumbuhan tanaman cabai merah di tanah masam. Metode yang digunakan adalah eksperimental dengan Rancangan Acak Lengkap (RAL) pola faktoial dengan dua faktor. Faktor pertama berupa dosis MVA campuran yang terdiri dari 0; 10; 15; 20 g/tanaman dengan kerapatan spora 10 7 spora/gram. Faktor kedua berupa dosis pupuk fosfat yang terdiri dari 0; 0,2; 0,4; 0,6 g/tanaman. Setiap kombinasi perlakuan diulang sebanyak 3 kali. Parameter yang diamati meliputi pertumbuhan tanaman (tinggi tanaman, diameter batang, berat kering tanaman bagian atas), derajat infeksi MVA, dan kandungan P jaringan tanaman. Data yang diperoleh dianalisis menggunakan Analisis Ragam (ANOVA) pada tingkat kesalahan 5% dan 1%, perlakuan yang berpengaruh signifikan atau sangat signifikan dilanjutkan dengan uji Beda Nyata Jujur (BNJ). Hasil penelitian menunjukkan interaksi MVA campuran dan pupuk P tidak dapat meningkatkan tinggi, diameter batang, dan berat kering bagian atas tanaman cabai, namun secara mandiri pemberian MVA campuran dan pupuk P dapat meningkatkan tinggi, diameter batang, dan berat kering bagian atas tanaman cabai merah. Dosis MVA campuran sebanyak 20 g/tanaman...
Tomato has an important role to fullfil the nutrition of society. The most important problem in the cultivation of tomatoes is fusarium wilt caused by Fusarium oxysporum it will attacking the plants from nursery to adult. One of the alternative control is use the Vesicles Arbuscular Mycorrhizae (VAM). The success of VAM infection in plants is determined by the dose and the inoculation. The aim of this research is to determine the effect of dose and mixture VAM inoculation to against the emergence of fusarium wilt in tomato plants and to determine the dosage mixture VAM inoculation as the most effective way for controlling fusarium wilt in tomato plants. The method of this research used experimental with completely randomized design. The experimental treatment consists of two types of treatment that are combined with used 5 doses of VAM mixture (0 g/plant, 10 g/plant, 12,5 g/plant, 15 g/plant, 17,5 g/plant) and used two ways of inoculation ( inoculation when the seed is planted and inoculation when transplanting the seeds). Each treatment was repeated 3 times and each test are three plants. The parameters was observed the incubation period of the disease and the intensity of fusarium wilt as the main parameter and the measurement of pH, temperature, humidity room, and the degree of infection as supporting parameters. The results of this research showed that the dosage and inoculation of VAM mixture is not able to reduce the emergence of fusarium wilt on tomatoes, but it was able to extend the incubation period of fusarium wilt on tomato plants a dose with 10 g /plant inoculated plants when the seeds are planted and inoculation when transplanting the seeds.
The aim of this study was to determine the effect of giving ArbuscularMycorrhizal Fungi (AMF), to the toxicity of Millet seeds (Foxtail millet) contaminated with batik waste water. This study used seeds contaminated with batik waste water with the addition of AMF and without AMF (negative control), as well as water-treated waste, Millet seeds that were not polluted with batik waste water with and without AMF added with distilled water (positive control). The results showed that AMF addition had an effect on reducing the toxicity of Millet seeds contaminated with batik waste water. Millet exposed to batik wastewater with the provision of AMF + water produced the best value of 57.78% germination, 42.22% inhibition, highest leaf length 1.13 cm, plumulae 1.89 cm, radics 1.58 cm. The provision of AMF is promising in preventing pollutants from entering plants as well as improving soil quality.
There are several interaction patterns of fungus that live in the rhizosphere of plants, i.e. mutualism, commensalism, saprophytism, and parasitism. The objective of this study was to determine the genera of microscopic fungus collected from the rhizosphere of groundnut, mays plant, and rice field. In addition, the diversity of soil fungi of those three plants was considered. Research method applied was survey with purposive sampling. The rhizospheric soil sample was obtained from research locations of groundnut, mays plants, and rice field in Purwokerto area. The soil samples were taken from 1-10 cm deep. Next they were isolated, and then they were purified. Furthermore, the obtained data of fungus collection were then analysed descriptively, and also described based on their macro and micro morphology. Then, the collected fungus were identified by using identification manual for fungus. The result showed that fifteen (15) isolates were found in three different rhizosphere of ground nut, mays plants, and rice field. The description is as follow: Aspergillus-1, Aspergillus-2, Penicillium-1, Penicillium-2, Mycophyta, Aureobasidia, Cylindrocarpon, Mucor-1, Mucor-2, Chaetomium, Gliocladium, Trichoderma-1, Trichoderma-2, Pleurophragmium, and Gonytrichum. Finally, the diversity Index (H’) noted from rhizosphere of groundnut plants was 0.312; mays was 0.195; and (H’) of rice field was 0.124.
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