Background
Today, when forensic experts talk about quantifiable hereditary traits, they do not just depend on the assessment and examination of DNA profiles but also relate them to the population structures. The use of high-throughput molecular marker technologies and advanced statistical and software tools have improved the accuracy of human genetic diversity analysis in many populations with limited time and resources. The present study aimed to investigate the genomic diversity in Gujarat’s Rabari population, using 20 autosomal genetic markers.
Numerous bio-statistical software programs are available for the interpretation of population data in forensics. These statistics deal with the measurement of uncertainty and also provides a probability of a random match. The present paper aims to provide a practical guide to the analysis of population genetics data. Three statistical software packages named Cervus, Genepop, and Fstat are compared and contrasted. The comparison is performed on the profiles obtained from fifty unrelated blood samples of healthy male individuals. DNA was extracted using the organic extraction method, 20 autosomal STR loci were amplified using PowerPlex 21 kit (Promega, Madison, WI, USA) and detected on 3100 Genetic Analyser (Life Technologies Corporation, Carlsbad, CA, USA).
Results
A total of 170 alleles were observed in the Rabari Tribe of Gujarat population, and allele frequencies ranged from 0.010 to 0.480. The highest allele frequency detected was 0.480 for allele 9 at locus TH01. Based on heterozygosity and the polymorphism information content, FGA may be considered as the most informative markers. Both the combined power of discrimination (CPD) and the combined power of exclusion (CPE) for the 20 analyzed loci were higher than 0.999999. The combined match probability (CPM) for all 20 loci was 2.5 × 10−22.
Conclusions
With respect to the results, the 20 STR loci are highly polymorphic and discriminating in the Gujarat population and could be used for forensic practice and population genetics studies. However, Fstat demonstrated better genetic software for analysis of the demographic structure of a specific or set of populations.
The present study aims to report genetic variations in unrelated male (n = 118) of the Gujarat population with the help of seventeen Y-STR markers (DYS456,
The aim of the study was to analyze unrelated samples from the Rewa male population of Central India by targeting Y-short tandem repeats (STR) and then compare the results to previously published Y-STR haplotype data.Materials and Methods. A total of 181 unrelated male subjects from the Rewa population were genotyped for seventeen Y-STRs (
Background: Stature estimation from body measurements is an important part of forensic and medico-legal cases especially in victim identifcation, where mutilated and amputated body parts are encountered at the crime scene. Hand and foot dimensions are the important features in context of stature estimation. The present study was conducted to find out the correlation of length and breadth of hand and foot in the stature of the North Indians. Methods: Anthropometric data from 413 males and 413 females were collected. Simple and multiple linear regression methods were used for estimation of stature from hand length, hand breadth, foot length and foot breadth measurements. Results: Statistically signifcant results (p <0.001) are obtained with each measurement when the derived model is tested for stature estimation. Hand length in both sexes showed highest correlation with the stature whereas foot breadth showed the lowest. Multiple regression models showed low standard error of estimate (SEE). Conclusion: The results of this study showed that, from a forensic stand point, measurements of the hands and feet may be employed to estimate the stature of North Indian populations.
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