A. ULRICH AND T. MÜ L LE R. 1998. Thirty-five strains of streptococci isolated from forage grasses were examined by restriction analysis of PCR-amplified 16S rDNA. Using a set of seven endonucleases, five 16S rDNA genotypes were obtained. The isolates could be identified as belonging to the species Enterococcus faecium, Ent. mundtii, Ent. faecalis, Ent. casseliflavus and Lactococcus lactis ssp. lactis, respectively. To assign the isolates to one of these species, digestion with the endonuclease Hinf I was sufficient. Data obtained from whole-cell protein analysis were in accordance with the species identification. Additionally, three of the five species found were subdivided into two related subgroups. Both methods are suitable for a rapid and unequivocal identification of plant-associated streptococci. The phenotypic characters showed a high diversity and were in some cases contrary to the expected features usually given in genus and species descriptions. The results demonstrated the close relatedness of the determined species in contrast to their high phenotypic heterogeneity.

Many kinds of industrial projects involve the use of prefabricated modules built offsite, and installation on-site using mobile cranes. Due to their costly operation and safety concerns, utilization of such heavy lift mobile cranes requires a precise heavy lift planning. Traditional heavy lift path planning methods on congested industrial job sites are ineffective, time-consuming and non-precise in many cases, whereas computer-based simulation models and visualization can be a substantial improving tool. This paper provides a Virtual Reality (VR) environment in which the user can experience lifting process in an immerse virtual environment. Providing such a VR model not only facilitates planning for critical lifts (e.g. modules, heavy vessels), but also it provides a training environment to enhance safe climate prior to the actual lift. The developed VR model is implemented successfully on an actual construction site of a petrochemical plant on a modular basis in which heavy lift mobile cranes are employed.

Abstract-The Pierre Auger Observatory is designed to unveil the nature and the origins of the highest energy cosmic rays. The large and geographically dispersed collaboration of physicists and the wide-ranging collection of simulation and reconstruction tasks pose some special challenges for the offline analysis software. We have designed and implemented a general purpose framework which allows collaborators to contribute algorithms and sequencing instructions to build up the variety of applications they require. The framework includes machinery to manage these user codes, to organize the abundance of user-contributed configuration files, to facilitate multi-format file handling, and to provide access to event and time-dependent detector information which can reside in various data sources. A number of utilities are also provided, including a novel geometry package which allows manipulation of abstract geometrical objects independent of coordinate system choice. The framework is implemented in C++, and takes advantage of object oriented design and common open source tools, while keeping the user side simple enough for C++ novices to learn in a reasonable time. The distribution system incorporates unit and acceptance testing in order to support rapid development of both the core framework and contributed user code.

Electrophoretic profiles of acid-extractable proteins from Holtzman rat liver chromatin display four minor and five major histone bands through certain stages of postweaned development but are qualitatively different from the chromatin protein profiles previously reported during postweaned development for the Fisher 344 rat strain and the F344 X Holtzman heterotic progeny [Tallman, G., et al. (1979). Biochem, Genet. 17:185]. The protein profiles from the heterotic progeny do not reflect and are not combinations of the profiles from the parental strains. Levels of in vitro transcription with Escherichia coli RNA polymerase of total chromatin and of acid-extracted chromatin from Holtzman rat liver tissue fluctuate in an age-specific manner during postweaned development and are higher than previously published levels determined for the Fisher and F344 X H strains during the same developmental period [Tallman, G., et al. (1978). J. Hered. 69:282]. The degrees of stimulation in the transcription assays resulting from the acid treatment vary with the age of the animal but are similar for the maternal Holtzman and hybrid strains. These studies suggest that regulation of heterotic growth may involve dominant, or maternal genetic influences.

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