The medicinal attributes of honey appears to overshadow its importance as a functional food. Consequently, several literatures are rife with ancient uses of honey as complementary and alternative medicine, with relevance to modern day health care, supported by evidence-based clinical data, with little attention given to honey’s nutritional functions. The moisture contents of honey extracted from University of Veterinary and Animal Sciences, Lahore honey bee farm was 12.19% while that of natural source was 9.03 ± 1.63%. Similarly, ash and protein contents of farmed honey recorded were 0.37% and 5.22%, respectively. Whereas ash and protein contents of natural honey were 1.70 ± 1.98% and 6.10 ± 0.79%. Likewise fat, dietary fiber and carbohydrates contents of farmed source documented were 0.14%, 1.99% and 62.26% respectively. Although fat, dietary fiber and carbohydrates contents of honey taken from natural resource were 0.54 ± 0.28%, 2.76 ± 1.07% and 55.32 ± 2.91% respectively. Glucose and fructose contents of honey taken out from honeybee farm were 27% and 34% but natural source were 22.50 ± 2.12% and 28.50 ± 3.54%. Glucose and fructose contents of honey taken out from honeybee farm were 27% and 34% but natural source were 22.50 ± 2.12% and 28.50 ± 3.54%. Similarly, sucrose and maltose contents of farmed honey were 2.5% and 12% while in natural honey were 1.35 ± 0.49% and 8.00 ± 1.41% respectively. The present study indicates that such as moisture, carbohydrates, sucrose and maltose contents were higher farmed honey as compared to the natural honey. In our recommendation natural honey is better than farmed honey.

During this one year study, blood and fecal samples of doves (Zenaida asiatica), ducks (Anas platyrhynchos), pigeons (Columba livia), partridges (Alectoris chukar), turkeys (Meleagris gallopavo) and goose (Chen caerulescens) were collected to assess the parasitic prevalence in these birds. The birds were kept at Avian Conservation and Research Center, Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore. All these avian species were kept in separate cages and their entire body was inspected on regularly basis to record external parasites. For internal parasites, 100 blood and 100 fecal samples for each species were analyzed. During present study, two species of ectoparasites i.e. fowl ticks (Args persicus) and mite (Dermanyssus gallinae) while 17 species of endoparasites; three from blood and 14 from fecal samples were identified. Prevalence of blood parasites was Plasmodium juxtanucleare 29.3%, Aegyptinella pullorum 15% and Leucoctoyzoon simond 13%. Parasitic species recorded from fecal samples included 6 species of nematodes viz. Syngamus trachea with parasitic prevalence of 50%, Capillaria anatis 40%, Capillaria annulata 37.5%, Heterakis gallinarum 28.3%, Ascardia galli 24% and Allodpa suctoria 2%. Similarly, two species of trematodes viz. Prosthogonimus ovatus having parasitic prevalence of 12.1% and Prosthogonimus macrorchis 9.1% were also recorded from fecal samples of the birds. Single cestode species Raillietina echinobothrida having parasitic prevalence of 27% and 3 protozoan species i.e. Eimeria maxima having prevalence 20.1%, Histomonas meleagridis 8% and Giardia lamblia 5.3% were recorded. In our recommendation, proper medication and sanitation of the bird’s houses and cages is recommended to avoid parasites.

Fermented cereal-based gruels with poor nutritional value form a major component of the diet of infants during the transition phase of childhood. With the recent security challenges affecting people in the north- east region of Nigeria, food security is at stake, malnutrition is common among infants and young children while reliance on UNICEF for supplies of ready- to- use therapeutic foods is not a long-term solution. A complementary food blend was prepared following the guideline of WHO/FAO on infants’ nutritional requirements in a 60:20:20 ratio from fermented yellow maize (improved variety), roasted soybean and fishmeal respectively. All the sample materials were pre-processed by either fermentation, drying or roasting prior to food blend formulation. Standard methods (AOAC) were used to evaluate the nutritional values of the raw and processed materials and the formulated complementary food blend. Water absorption capacity of the fermented yellow maize significantly decreased (P< 0.05) with decrease in pH and increase in titratable acidity from 20% - 51% (0 – 72h). The carbohydrate (64.35±0.03%) and protein contents (14.55±0.03% and energy value (351.64±0.03 Kcal/100g) of the complementary food blend was closely comparable to the commercial complementary food blend cerelac® (Carbohydrate 69.00%, Protein 15.0%, and energy 426.00 Kcal/100g) and satisfied the needs of infants. The low moisture content (3.1±0.02%) exhibited by the complementary food blend might give a good storage stability. The iron level of the complementary food blend (40.33± 0.03%) was higher than that of the commercial complementary food blend celerac® (10.0%), though the levels of potassium, zinc and calcium were lower. Microbial analysis showed no contamination with pathogenic organisms in the formulated food blend. The formulated complementary food blend met the WHO estimated requirements of infant 6 – 23 months in terms of nutritional quality and may therefore be a good substitute to the imported, expensive alternatives.

The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these protozoans, Cryptosporidium considered being one of the important water-borne zoonotic pathogens and a major cause of a diarrheal disease named cryptosporidiosis in humans, domestic animals, and wild animals. This study was aimed to identify Cryptosporidium in zoo felids (N= 56) belonging to different zoo of China, but accidentlly Colpodella was encountered in the zoo felids sample and phylogenetic data confirmed this unexpected amplification from fecal samples using two-step nested-PCR. Phylogenetic analysis revealed the fact about the specific primers used previously by many researchers and cross-genera amplification. We came to know that genetically sequenced amplicon gives more accurate identification of species. This study suggests more investigation on Colpodella which has been neglected previously but gains the attention of researchers after identified from humans and animals and has been known to correlate with neurological symptoms in patients.

Chemical fertilizers are crucial in the production of cost-effective agricultural crops. However, long-term usage of chemical fertilizers will deteriorate the soil quality and it is hazardous to human health. Scientists and researchers across the globe are seeking the help of nanotechnology as a possible solution to combat the hazardous effect of chemical fertilizers. Nanotechnology is a branch of science and engineering concerned with the matter at the nanoscale or one billionth of a meter. Nanofertilizers are modified fertilizers that are synthesized using techniques of nanotechnology involving various physicochemical and biological methods. These methods aid in enhancing their attributes and composition, which leads to a positive effect on crop productivity. Nanofertilizers are far more beneficial when compared to chemical fertilizers as the former are cost-effective, less toxic and show controlled and regulated release of nutrients to plants. This chapter is primarily concerned with the various methods employed in nanofertilizer synthesis, the economic importance of nanofertilizers and their advantage over conventional chemical fertilizers.&nbsp;<br>