Urolithiasis is the urinary disorder characterized by formation of stone in the urinary tract, urinary bladder and kidneys. Urolithiasis is considered to be a risk of urinary incontinence and may be associated with urinary incontinence. A urinary stone commonly occurs in males and are calculated on the basis of their mineral composition. Approximately 80% of urinary stones composed as calcium oxalate and calcium phosphate. Urinary calculi can cause disruption to the urinary system, hydrophoresis, infections and bleeding. Root stone formation is the result of several mechanisms. Whereas exceeding supersaturation (i.e. free stone formation) is the cause of uric acid or cystine calculi, infection stones result from bacterial metabolism. Renal calculi can be broadly classified in two large groups: tissue attached and unattached. Components in diet increase the risk of stone formation include low intake of fluid and animal protein, sodium, sugar and syrup high corn fruit, oxalate, grape juice, apple juice and cola drinks.   Vegetarian diets, spices and liquids may be helpful in treating and prevention and kidney stones. Therefore, the best way to prevent kidney stones is to consume a lot of water and a magnesium rich vegan. Common medications used to prevent urolithiasis do not work for all patients, and many have adverse effects that endanger their long-term use.  Managing today's urolithiasis with open renal surgery is unusual and rarely used, only by the introduction of Extracorporeal Shock wave lithotripsy (ESWL) is useful but it is expensive. Herbs and herbal medicines have created interest among people with its clinically proven effects such as immunomodulation and antimutagenic. Also, abusing synthetic drugs due to serious side effects of the drug has allowed humans to return to nature for safe treatment. Kew words: hydrophoresis, Urolithiasis

Introduction: Renal stone formation and the predominant chemical stone composition are age and gender dependent. The most common stones are struvite (magnesium ammonium phosphate), calcium oxalate, urate, cystine and silica. The most common type of kidney stones worldwide contains calcium. The process of forming stones in the kidney, bladder, and/or urethra (urinary tract) is called as Urolithiasis. Urinary calculi may cause obstruction, hydronephrosis, infection and hemorrhage in the urinary tract system. Citrus species were used in traditional practices as remedies for the urolithiasis have been well established in vivo-vitro test, clinical trial and effective use but are lacking well documented record. Citrus limon (L.) is tree with evergreen leaves and yellow edible fruits from the family Rutaceae. Citrus aurantiumL commonly called as bitter orange. Citrus medica Linn.,commonly known as a Citron in English and bijapura in Ayurvedic literature is shrub or small tree. All three are reported to use in treatment of wide panel of diseases like antiurolithic activity, stomach ache, vomiting antifungal and antibacterialetc. Urolithiasis was introduced by administration of ethylene glycol in rats. Standard drug treated group, the urinary outputs increased significantly. Body weight of all animals measured on day 0, 14 and 28 of calculi induction to assess the effect of treatment on body weight. The collection of urine from rats scheduled to be every seven days from calculi induction. C. aurantinum extract showed significantly increase in urinary output. The collected urine stored in 4ºC. The analysis of calcium, phosphate, magnesium, creatinine, uric acid, protein, urea nitrogen and oxalate recorded.300 mg/kg of combined extract of all three plants show significant result compare to individual plant response.

The present investigation was designed to evaluate the anti-asthmatic activity of ethanolic extract of Murraya Koenigii Leaves in experimental animals. Murraya Koenigii Leaves was evaluated for anti-histaminic activity using isolated Guinea pig tracheal chain preparation, histamine induced Bronchoconstriction in Guinea pig and milk induced leucocytosis in mice. Ethanolic extract of Murraya Koenigii Leaves oral dose 400mg/kg body weight significantly inhibited dose dependent contraction of Guinea pig tracheal chain produced by histamine and also showed significant protection by prolonging PreConvulsion Dyspnoea time (PCT) in guinea pigs. Murraya Koenigii Leaves was evaluated by milk induced leucocytosis in mice showed significant decreased of total leukocyte count. Thus, Murraya Koenigii Leaves showed anti-allergic activity against histamine hence possesses potential role in the treatment of asthma.

Antioxidant play an important role to protect damage caused oxidative stress (OS).Chlorophyll having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties of methanolic solution from Super Chlorophyll. Objective: The main objective of the study was to evaluate the antioxidant activity of the chlorophyll in different validated in vitro models. Methods: The antioxidant activities of methanolic solution were evaluated by in vitro standard method using UV-Spectrophotometer. The antioxidant activity were determined bt total antioxidant capacity, DPPH (1-1-diphennyl-2-picrylhydrzine) radical scavenging assay, Nitric Oxide scavenging assay and Hydrogen Peroxide scavenging assay methods. Result: The solution of Chlorophyll was studied for antioxidant potential. Ascorbic acid was used as standard. This method is simple and activity of the solution is reported in term of IC50 value. Antioxidant properties depend on the IC50 value. Lesser the IC50 value, more is the antioxidant activity. Chlorophyll showed good antioxidant activity. Conclusion:  In case of in vitro antioxidant activity, Chlorophyll scavenged DPPH, hydrogen peroxide, nitric oxide radicals significantly, showed IC50 values near to standard ascorbic acid, thus proving to have good antioxidant potential. Keywords: Oxidative stress, DPPH, Antioxidant, Super chlorophyll.