Introduction: The SNP HLA-C-35 kb (rs9264942) may contribute to the host immune defense mechanism by affecting the cell surface expression pattern of HLA-C and antigen presentation to CD8+ cytotoxic cells. Thus, this SNP may contribute to intracellular multidrug-resistant (MDR)-tuberculosis (TB) infection. Aim:To examine the association between the SNP HLA-C-35 kb (rs9264942) and the clinical profile of MDR-TB infection.Settings and design: MDR-TB-positive patients were followed from May 2012 to December 2013 to observe the progression of MDR-TB infection. Non-TB individuals and non-MDR-TB individuals were also recruited as controls. Materials and Methods:The patients' HLA-C-35 kb (rs9264942) status was determined by PCR.results: The C allele was slightly more frequent in the MDR-TB patients than in the non-MDR TB patients (OR= 1.28; 95% CI: 0.701 -2.328). The C allele was found to be more frequent in the MDR-TB patients exhibiting pulmonary fibrosis (OR= 2.13; 95% CI: 0.606 -7.480) or pulmonary infiltrates (OR= 3.17; 95% CI: 0.690 -14.598) and among the MDR-TB patients who were classified as underweight (OR= 8.00; 95% CI: 1.261 -50.770). The CC genotype was associated with the treatment after failure of category II group (OR= 4.17; 95% CI: 1.301 -13.346). conclusion: The C allele SNP HLA-C-35 kb (rs9264942) may contribute to the clinical profile in MDR-TB infection.