Late state‐of‐the‐art analytical methodologies in chromatography, spectroscopy, and electroanalysis have been developed to meet the challenges of changing environmental and health issues. The modern trends in developing new protocols emphasize economic, portable, nano, or even smaller sample sizes and less time‐consuming processes. This has led to the development of technology‐based biosensors which meet most of the above requirements. The lab‐on‐chip technology exploiting enzyme‐based biosensors has made the analytical processes very efficient, accurate, affordable, and requiring nano‐scale sample sizes. In this review, an attempt is being made to review the literature based on state‐of‐the‐art technology of enzyme‐based biosensors for the detection of biomolecules.

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An easy, inexpensive, and accurate method for the analysis of synthetic dyes by means of ion-pair thin-layer chromatography (IPTLC) on mixed sorbent phases containing silica gel G and barium sulfate in an aqueous ethanol system is studied. The effect of the composition of the sorbent phases has a major effect on the hundred-fold relative migration rate, as also does the effect of the ion-pairing reagent as the impregnant. Compact and sharp spot application yields very good binary and ternary separations and enables their clear identification. IPTLC has better separations on mixed sorbent phases, which are not possible on plain mixed phases in thin-layer chromatography. The method can be applied for the trace analysis of synthetic dyes in various natural and synthetic samples.

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