The aim of the study was to evaluate prognosis for biochemical recurrence (BR) by analysing the pathological and biological characteristics of prostate cancer (PCa) after radical prostatectomy (RP). There were 130 men with clinically localized PCa in whom pretreatment serum PSA level and Ki-67, prostate specific membrane antigen (PSMA), glucose transporter-1 (GLUT-1), vascular endothelial growth factor (VEGF), microvessel density (MVD) and human telomerase reverse transcriptase (hTERT) proteins expression, based on number of immunohistochemically positive cells (labelling index), were retrospectively studied. In order to assess the prognostic significance of analysed variables in univariate and multivariate Cox analysis, patients were dichotomized based on cutoff points chosen by receiver operating characteristic (ROC) curves. There were 83 males (63.8%) at pT stage 1-2 and 47 (36.1%) at pT stage 3-4, respectively, with median (range) age of 62.8 years (49-77), and median followup of 78.5 months (12-148). In 42 (32.3%) men BR was found. In univariate analysis, tumour biological features: PSA ≤ 8 ng/ mL (p = 0.006), Ki-67LI ≤ 12.7% (p = 0.015), VEGFLI>11.0% (p = 0.030), and hTERTLI>6.7% (p = 0.016), but not clinicopathological parameters, appeared to be positive prognosticators for BRFS. In the Cox analysis, Ki-67 lost its significance, and clinicopathological parameters appeared to be nonsignificant. The independent negative prognostic factors for BRFS were: PSA > 8.0 ng/mL, (Hazard ratio = 2.75, p = 0.003), GLUT-1 > 19.1% (HR = 2.1, p = 0.032), VEGF≤11.0% (HR = 1, p = 0.024) and hTERT≤6.7% (HR = 1, p = 0.017). High PSA level, and GLUT-1 expression and lower VEGF and nuclear hTERT expression may indicate the great role of hypoxia in BR induction in PCa.

Diagnostic utility of HE4 was similar to that of NSE and ProGRP. Complementary to NSE, determination of HE4 seems to be helpful in evaluation of SCLC patients' prognosis.

Acute exercise-induced changes in cortisol concentration (C) and training related adaptation within hypothalamic-pituitary-adrenal (HPA) axis has been widely examined, but their influence on muscle strength performance is at best uncertain. Twenty four young healthy men were randomly assigned to an endurance training group (ET, n=12) or to a non-exercising controls (CON, n=12). ET performed supervised endurance training on cycle ergometer for 20 weeks. Endurance training program improved exercise capacity (14 % increase in power output generated at peak oxygen uptake – VO2peak), muscle strength performance (increase in MVC – maximal voluntary contraction – by 9 % and in TTF 50 % MVC – time to fatigue at 50 % MVC – by 21 %) and led to a decrease in basal serum C concentration (P=0.006) and an increase in basal testosterone to cortisol (T/C) and free testosterone to cortisol (fT/C) ratios (P=0.01 and P=0.02, respectively). It was found that the decrease in C concentration (ΔC) was positively correlated to the increase in local muscular endurance (ΔTTF 50 % MVC). No significant hormonal changes were seen in CON group. It is concluded that greater decrease in cortisol concentration after the endurance training is accompanied by poorer improvement in skeletal muscle performance in previously untrained subjects.

No abstract

Purpose: The aim of the study was to evaluate morphological, biochemical, and rheological blood indicators in men staying in a cryochamber at a temperature of -50°C for 24 hours. In 2018, a scientific-survival project ‘Taming the Frost’ was conducted at the Technoclimatic Research and Working Machines Laboratory of the Cracow University of Technology, under the scientific patronage of the Rector of the Cracow University of Technology, Prof. Jan Kazior, PhD, as well as the Rector of the University of Physical Education in Krakow, the late Prof. Aleksander Tyka, PhD, and the Vice-Rector for Science, Prof. Anna Marchewka, PhD. Material and methods: The blood for the tests was collected from an ulnar vein in fasting participants by a qualified nurse, in the morning, before entering the cryochamber and after 24 hours, i.e. on leaving the cryochamber. Morphological, biochemical, and rheological blood indicators were evaluated. The study group of the scientific-survival project ‘Taming the Frost’ involved men (n=6) who stayed in a cryochamber at a temperature of -50°C for 24 hours. For each participant, a 5-ml blood sample was placed in a tube (BD Vacutainer) with EDTA K2 anticoagulant for blood morphology and blood rheological evaluations in the Blood Physiology Laboratory of the Central Research and Development Laboratory, University of Physical Education in Krakow. Another 5-ml blood sample was placed in a Vacuette tube with a clotting activator for the remaining biochemical analyses in the Department of Clinical Biochemistry of the Krakow Branch of Maria Skłodowska-Curie National Research Institute of Oncology. Results: After leaving the cryochamber with a temperature of –50°C, the participants presented statistically significant increases in monocyte count and high-density lipoprotein and creatine kinase values, as well as decreases in IgA, total cholesterol, and triglycerides. In the assessment of blood rheological indicators, statistically significant increases in the elongation index at the shear stress of 0.30, 0.58, 1.13, and 2.19 Pa and decreases in the elongation index at the shear stress of 31.03 and 60.3 Pa were observed. For red cell aggregation indicators, a statistically significant increase in total aggregation time was reported. The other indicators exhibited a significance level of p>0.05. Conclusions: Staying in a cryochamber at a temperature of -50°C for 24 hours did not exert a negative impact on morphological, biochemical, or rheological blood indicators, which implies the subjects’ adaptation to the arranged conditions.