Safety assessment is reported of an orally ingested dihydroquercetin-rich extract (Lavitol) derived from the Dahurian larch tree, used as a food additive and as a dietary supplement ingredient. Dihydroquercetin, a potent antioxidant, is also known as taxifolin. The results of genotoxicity and toxicological tests (Comet assay, micronucleus test in human lymphocytes, chromosomal aberration test, subacute 7-day oral toxicity study, subchronic 90-day toxicology study with histopathologies, and, prenatal and postnatal developmental toxicity studies) on the extract provide further support for the safety of its consumption as a food supplement and food additive.
In this research, we present a detailed comparative analysis of the bioactive substances of soybean varieties k-11538 (Russia), k-11559 (Russia), k-569 (China), k-5367 (China), k-5373 (China), k-5586 (Sweden), and Primorskaya-86 (Russia) using an LSM 800 confocal laser microscope and an amaZon ion trap SL mass spectrometer. Laser microscopy made it possible to clarify in detail the spatial arrangement of the polyphenolic content of soybeans. Our results revealed that the phenolics of soybean are spatially located mainly in the seed coat and the outer layer of the cotyledon. High-performance liquid chromatography (HPLC) was used in combination with an amaZon SL BRUKER DALTONIKS ion trap (tandem mass spectrometry) to identify target analytes in soybean extracts. The results of initial studies revealed the presence of 63 compounds, and 45 of the target analytes were identified as polyphenolic compounds.
Research is devoted to the study of a metal/carbon nanocomposite of copper as a stimulator of root formation during vegetative propagation of the Mock-orange (Garden jasmine) hybrid ( Philadelphus x hybridus hort.) of cultivars Komsomolec and Snezhnaja Burja, western Thuja (Northern whitecedar) of cultivar Pyramidalis Compacta ( Thuja occidentalis L. Pyramidalis Compacta). Using the example of green cuttings of ornamental crops at all concentrations of a finely dispersed suspension of metal/carbon nanocomposite of copper (TDS Me/C NC), a stimulating effect on their survival is shown. In the variants of treatment of cuttings with a NС suspension of 0.05, 0.5 and 1.0 % concentration, 68.2, 72.8 and 65.4 %% of cuttings rooted, which was 18.4, 23.0 and 15.6 %%, respectively, more than in the control (water) (НСР = 7.8).
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