Research is devoted to the study of a metal/carbon nanocomposite of copper as a stimulator of root formation during vegetative propagation of the Mock-orange (Garden jasmine) hybrid ( Philadelphus x hybridus hort.) of cultivars Komsomolec and Snezhnaja Burja, western Thuja (Northern whitecedar) of cultivar Pyramidalis Compacta ( Thuja occidentalis L. Pyramidalis Compacta). Using the example of green cuttings of ornamental crops at all concentrations of a finely dispersed suspension of metal/carbon nanocomposite of copper (TDS Me/C NC), a stimulating effect on their survival is shown. In the variants of treatment of cuttings with a NС suspension of 0.05, 0.5 and 1.0 % concentration, 68.2, 72.8 and 65.4 %% of cuttings rooted, which was 18.4, 23.0 and 15.6 %%, respectively, more than in the control (water) (НСР = 7.8).
The research is devoted to the development of clonal micro-propagation technology of the decorative cereal Molinia caerulea (L.) Moench. The concentration of 2.0 mg /l of cytokinin 6-benzylaminopurine (6-BAP) contributed to obtaining the maximum number of microshoots: 6.3 and 7.9 pcs. on Anderson's and Murashige-Skoog's (MS) media, respectively, which exceeded the control (by 4.7 and 6.3, respectively, with LSD05 = 2.3). The length shoots were observed on hormone-free media, this indicator significantly decreased with an increase in 6-BAP content. On Anderson and MS media with 1.0 mg/l 6-BAP, the shoot length averaged 21.5 and 26.4 mm, respectively, which made it possible to transplant them for rooting, bypassing planting on a medium for elongation. The inclusion of the Siliplant micro-fertilizer in the MS medium at doses of 1.0 and 2.0 ml/l contributed to a significant increase in shoot size, by 16.7 and 10.7 mm (LSD05 = 8.9), respectively, in comparison with the control (MS). It is recommended to use Anderson's medium and 0.5 mg/l of indole-3-acetic acid as a medium for rhizogenesis: after two weeks of cultivation, the regenerants had a standard appearance with developed roots suitable for planting for adaptation. At the adaptation stage, watering the substrate with the biofungicide «Trichoderma veride» according to the instructions and a cereals single spraying with the micro-fertilizer «Siliplant» at a dose of 1.5 ml/l contributed to their 100 % survival rate.
Использование декоративных особенностей цветочных растений позволяет создавать высокохудожественные цветочные композиции. К числу основных проблем относятся «бесцветочный» весенний период, пустующие затененные территории, однообразие стилевого направления, широкое распространение дорогостоящих клумб из растений однолетников, их ограниченный ассортимент. Создание в условиях городской среды устойчиво-декоративных цветников в течение всего вегетационного периода должно опираться на 3 определяющих фактора: экологический, эстетический и экономический. Проанализирован видовой состав цветников, дана комплексная оценка декоративности и устойчивости многолетних травянистых декоративных культур в условиях Среднего Предуралья. Выявлено, что специализированные объекты цветочного оформления — массивы, группы, отдельные растения, объединенные композиционным замыслом, включали в себя многолетние культуры 74 % растений (28 видов) и имели высокую декоративность — 13 баллов из 13 возможных. Многолетники даже со слабым агротехническим уходом имели высокую оценку качества и декоративности, устойчивы к данным климатическим условиям, произрастают на одном месте 3 – 5 лет и более, являются более экономичными по сравнению с однолетними цветочными культурами.
The paper presents the results of 2018-2019 research on improving the technology of growing planting material of bog cranberry (Vaccinium oxycoccos L.) of Krasa Severa, Severyanka, Virussaare varieties on the basis of in vitro. Studied was the effect of the concentrations of growth regulators in the composition of the nutrient medium according to Anderson's recipe on the reproduction and subsequent rooting of micro cuttings, as well as the duration of cultivation and adaptation of micro plants depending on partial pruning of shoots. It has been established that at the stage of introduction into in vitro culture, sterilization of explants with 33% hydrogen peroxide in an exposure of 5-8 minutes with washing in 5 portions of sterile distillate gives 60-80 % of viable shoots. The optimum phase of plant development for the successful introduction of in vitro culture is the swelling of buds. Cultivation of micro cuttings was carried out in a light room at a temperature of 25±2 °С, a photoperiod of 16 hours. The duration of each subculturing was 30-60 days. For the stage of actual micropropagation on Anderson's nutrient medium, an increase in the dose of cytokinin 6-benzylaminopurine (6-BAP) from 0.2 to 0.5 mg/l and an increase in the duration of cultivation from 30 to 60 days contributed to a significant increase in the multiplication factor on average for the tested cranberry varieties.According to the efficiency of micropropagation, the varieties Virussaare and Krasa Severa were distinguished – 9.3-12.0 pcs/stalk, respectively. At the rooting stage, the use of a root-forming reagent of indolyl-3-acetic acid (IUK) in doses of 0.2, 0.5 and 1.0 mg/l in the composition of Anderson's nutrient medium did not affect the quality of root formation and the length of shoots of Virussaare micro-plants. No significant varietal differences in the root-forming ability of microcuttings were found. The tendency of better rooting of micro cuttings was observed in the Virussaare variety (90.3 %) compared to the Severyanka (85.7 %) and Krasa Severa (79.3 %) varieties. Micro plants of the Krasa Severa cultivar were characterized by the longest shoots, the total number of roots was less, but their length was longer in comparison with other cultivars. For the adaptation stage, a substrate from a mixture of lowland peat and sphagnum moss was used (1:1). The efficiency of adaptation of micro plants of cranberry varieties when cutting the tip of the shoots was 100 %. Pruning of micro plants shoots contributed to the formation of more side shoots and better development of the aboveground part of the plants.
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