Using two photocolorimetrical methods described in the literature the authors carried .out a sequential study for the quantitative estimation of hellebrin in Helleborus purpurascens. The defating of the.plant material in a Soxhlet was faster and more reliable. The drug may be considered exhausted by three extractrions with methanol as solvent and a drug-solvent ratio of 1 :20. The method indicated by the literature and based on a chloroform-methanol extraction of the first hydromethanolic extractive solution was not completly operative. Hellebrin was separeted by thin layer chromatography. The obtained residue after the elimination of the solvent was used, and the colour reaction with p-dimethylaminobenzaldehyd was carried out directly on silicagel. By heating on a boiled water bath for 15-20 minutes, the most strong and stable colour was obtained.

No abstract

The pure saponins of Primula officinalis and Primula elatior, the primulic and the elatioric acid, were obtained chromatographically and compared in order to establish if they could be considered as be same substance. Comparing their hydrolysis by-products and ZR spectres there was painted out that the primulic and elatioric acids aren't identical. Zusammenfassung Zur Klarung der Frage, ob Prirnulasaure und Elatiorsaure identische Substanzen sind, wurden die beiden Saponine chromatographisch rein aus Wurzeln der Primula officinalis (L.) HILL. und ~r i m u l a elatior (L.) GRUFB. nit sicheren Herkiinften gewonnen. Aus dem Vergleich der Hydrolyseprodukte und der IR-Spektren konnte gefolgert werden, da/3 sie keine identische Substanzen sind. Die Hauptsaponine aus den Wurzeln von Prirnula officinalis (L.

Durch zahlreiche Studien sind die Unterschiede im Alkaloidgehalt von Solanaceen in den verschiedenen Pflanzenorganen wie auch im Verlauf der ~e~e t a t i o n speriode bekannt. Angaben iiber diese Veränderungen, speziell für Datura innoxia, gibt es hingegen verhältnismäRig wenige.Hinsichtlich der Verteilung der Gesamtalkaloide in den einzelnen Organen stiinmeri sämtliche Untersuchungen darin iiberein, dag die reichhaltigsten Pflanzenteile die unreifen Früchte, die Samen und die Wurzeln sind. Es folgen die Blätter und die Stengel, bei denen wiederum der Gehalt um so höher liegt, je jünger sie sind ( G e r a s i m e n k o , 1960; K r a s o w s k a , 1961; V a g u j f a l v i , 1963; V e r z a r -P e t r i , 1966). Bei diesen Organen sind die Abweichungen zwischen den einzelnen Etagen beträchtlich. Tiefster und höchster Gehalt können um 400-600% differieren. ( M a t e e s c u U.

Three glycosides of 1,s-dihidroxy-2-acetonaphtone were isolated from bark o f Rhamnzls frangula. T h e glucidic rest of the first was gltrcose and rhamnose while in the two others it was only glticose. It has been established that in the two natural glzicosides the glticidic rest was bound in diglucidic form to the one of C1 or C8 hydroxyl and the two nattiral glucosides were positional isomers. This was proved by quantitative estimation of aglyconlglucoside ratio, throzfgh preparation of monomethylether as well as comparing to the semisynthesis 1,8-dihidroxy-2-acetonaphtone of the 1,8-diglucoside.