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Background:Recently, tissue engineering has developed approaches for repair and restoration of damaged skeletal system based on different scaffolds and cells. This study evaluated the ability of differentiated osteoblasts from adipose-derived stem cells (ADSCs) seeded into hydroxyapatite/tricalcium phosphate (HA-TCP) to repair bone.Methods:In this study, ADSCs of 6 canines were seeded in HA-TCP and differentiated into osteoblasts in osteogenic medium in vitro and bone markers evaluated by reverse transcription polymerase chain reaction (RT-PCR). Scanning electron microscopy (SEM) was applied for detection of cells in the pores of scaffold. HA-TCP with differentiated cells as the test group and without cells as the cell-free group were implanted in separate defected sites of canine's tibia. After 8 weeks, specimens were evaluated by histological, immunohistochemical methods, and densitometry test. The data were analyzed using the SPSS 18 version software.Results:The expression of Type I collagen and osteocalcin genes in differentiated cells were indicated by RT-PCR. SEM results revealed the adhesion of cells in scaffold pores. Formation of trabecular bone confirmed by histological sections that revealed the thickness of bone trabecular was more in the test group. Production of osteopontin in extracellular matrix was indicated in both groups. Densitometry method indicated that strength in the test group was similar to cell-free group and natural bone (P > 0.05).Conclusions:This research suggests that ADSCs-derived osteoblasts in HA-TCP could be used for bone tissue engineering and repairing.

Introduction:Lifestyle improvement is one of the main strategies to increase treatment persistence in breast cancer survivors. The purpose of the present study was to determine the effect of a light-intensity physical activity program on Socio-cognitive factors that affect sedentary behavior and lifestyle in breast cancer survivors in Isfahan city.Methods: Twenty-four female breast cancer survivors were recruited via convenience sampling and randomly assigned to experiment and control groups (12 subjects for each group). Both groups were assessed using Busschaert's context-specific sedentary behavior questionnaire and the Miller-Smith Lifestyle Assessment Inventory before and after the intervention. The light-intensity physical activity program was conducted for one month in the experimental group based on Australia's Physical Activity and Sedentary Behavior Guidelines for Adults. Data were analyzed using descriptive statistics including mean and standard deviation and inferential statistics including ANCOVA and MANCOVA with a significance level of 0.05. Results:There was a significant difference between the two groups in lifestyle and Sociocognitive factors affecting sedentary behavior (except for the sedentary behavior modelling) (p= 0.001). Conclusion:It seems that performing light physical exercises may improve the lifestyle and Socio-cognitive factors affecting sedentary behavior by changing key factors such as motivation and intrapersonal relationship in breast cancer survivors.