Bone repair with differentiated osteoblasts from adipose-derived stem cells in hydroxyapatite/tricalcium phosphate in vivo

Abstract

Background:Recently, tissue engineering has developed approaches for repair and restoration of damaged skeletal system based on different scaffolds and cells. This study evaluated the ability of differentiated osteoblasts from adipose-derived stem cells (ADSCs) seeded into hydroxyapatite/tricalcium phosphate (HA-TCP) to repair bone.Methods:In this study, ADSCs of 6 canines were seeded in HA-TCP and differentiated into osteoblasts in osteogenic medium in vitro and bone markers evaluated by reverse transcription polymerase chain reaction (RT-PCR). Scanning electron microscopy (SEM) was applied for detection of cells in the pores of scaffold. HA-TCP with differentiated cells as the test group and without cells as the cell-free group were implanted in separate defected sites of canine's tibia. After 8 weeks, specimens were evaluated by histological, immunohistochemical methods, and densitometry test. The data were analyzed using the SPSS 18 version software.Results:The expression of Type I collagen and osteocalcin genes in differentiated cells were indicated by RT-PCR. SEM results revealed the adhesion of cells in scaffold pores. Formation of trabecular bone confirmed by histological sections that revealed the thickness of bone trabecular was more in the test group. Production of osteopontin in extracellular matrix was indicated in both groups. Densitometry method indicated that strength in the test group was similar to cell-free group and natural bone (P > 0.05).Conclusions:This research suggests that ADSCs-derived osteoblasts in HA-TCP could be used for bone tissue engineering and repairing.

Whey Protein Isolate/Calcium Silicate Hydrogels for Bone Tissue Engineering Applications—Preliminary In Vitro Evaluation

“…The primer's sequences for Col I, bALP, and GAPDH were projected using the Primer-Blast tool [24]. The primer sequences for OC were developed based on data from the literature [25].…”

Cytotoxicity assessment of polyhydroxybutyrate/chitosan/nano- bioglass nanofiber scaffolds by stem cells from human exfoliated deciduous teeth stem cells from dental pulp of exfoliated deciduous tooth

“…[ 19 20 ] For this reason, biologically active materials such as hydroxyapatite, nano-bioglass (nBG), collagen, or gelatin in combination with chitosan have been developed to improve their mechanical or biological properties. [ 21 22 23 24 25 26 ] Bioactive glasses due to their ability in binding to the surrounding tissues have been widely studied so far. The produced glasses in this way have good conduction and induction of ossification.…”

Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP—Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats

“…An ideal bone substitute must be biocompatible, osteconductive to enable osseointegration, and osteoinductive to stimulate the mesenchymal stem cells to differentiate in bone-forming cells [11,12], Additionally, they must also undergo the degradation and resorption processes in vivo [13,14]. These relevant characteristics have been identified in several studies dealing with CaPs such as β-tricalcium phosphate (Ca 3 (PO 4 ) 2 , β-TCP) [15,16,17,18,19,20,21] and hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , HA) [15,16,17,19]. The biodegradation mechanisms and resorption behavior in vivo of CaPs materials intended for bone repair and tissue engineering applications were recently reviewed [3,13].…”