Studies on potato (Solanum tuberosum L.) plants have been carried out to plant tissue culture laboratories because of the disease and virulent free seed tubers in field conditions. Since generative production in commercial potato production is not economically massive, production is made more economically with tubers. Jasmonic acid (JA) and its related compounds are newly recognized plant growth regulators and they are known to have effects on many physiological processes. In this study, it was aimed to determine the effects of methyl jasmonate and sucrose on micro tuber formation in potato micropropagation. To serve the purpose the relationships between twelve media combinations (including control medium), varying concentrations of sucrose (3, 6 and 9 %) either alone or in combination with 1.0 ppm or 2.0 ppm methyl jasmonate were investigated. Three different plant parts such as node, shoot eye and primordial tissue wereused as explant sources. Nitsch&Nitsch was used as the basic nutrient medium and plant growth regulators that used in media were constant at 2.5 mg/L BAP + 0.5 mg/L NAA except for control medium. Obtained micro tubers, plantlets and callus formations were recorded and analyzed. Results revealed that, when methyl jasmonate used at 1.0 ppm concentration in media, it had a positive effect on micro tuber formation and can be recommended to use commercially in mass production.
In this study, 90 locally grown cucumber (Cucumis sativus L.) landraces were collected and morphologically characterized using 20 descriptors derived from UPOV (International Union for the Protection of New Varieties of Plants). Genetic diversity and relationships of the genotypes were revealed using 20 sequence-related amplified polymorphism (SRAP) marker combinations. The discrimination power of each polymorphic marker (estimated by the polymorphism information content) ranged from 0.15 to 0.99 with an average of 0.73. Dice's similarity coefficient ranged between 0.00-1.00. The cluster analysis that was conducted using the unweighted pair group method of arithmetic averages (UPGMA) for both molecular and morphologic data showed that all of the genotypes fell into two main groups and many subdivisions. According to morphological data, fruit length, diameter and weight of the genotypes were determined between 6.5 - 32.5 cm, 25 - 52 mm and, 28 - 625 g respectively. It is clear from the results, a moderate level of genetic diversity, which has the potential for broadening the genetic base, was observed among the Turkish cucumber landraces.
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