Clinically nonphlogogenetic phagocyte reaction under conditions of bacterial challenge was studied in vivo. The "mission" of phagocytes under such conditions is completed by evacuation of phagocytized bacteria from the site of capture into the blood and then into the intestine. The purulent process induced by massive doses of Staphylococcus aureus (25• 106 and 25x 108 bacteria), without any concomitant injury to the peritoneum does not lead to the development of inflammation.

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Antibacterial activity of neutrophils and peritoneal macrophages toward massive doses of Staphylococcus aureus is studied in vivo. Two types of antibacterial response are revealed: nonphlogngenie (physiological) and phlogogenic (inflammatory). Nonphlogogenic reaction is characterized by pronounced ant~aeterial effect of phagocytes on cocci. Transition to phlogogenic response is accompanied by impaired function of phagocytes involving their self-destruction and disintegration, which decreases their antibacterial activity and promotes inflammation.Key Words: neutrophil; staphylococcus; macrophage; phagolysosome Despite extensive research, general pathological concept of inflammation has not been formulated. All conclusions concerning the function of phagocytes are based on the investigation of inflammatory reaction [1,3,9,10].Recent studies deal with nonphlogogenie reactions of neutrophilic leukocytes (NL), i.e., their functioning under physiological conditions [2,[6][7][8]. It was demonstrated that the system of NL effectively reacts under physiological conditions to rather strong bacterial attacks. The functioning of the system in this case is intensive, i.e., a small amount of cells (about 13%) are involved in antibacterial response; phlogogenic reaction is extensive [3,7].We studied morphological changes of St. aureus phagocytized by NL and peritoneal maerophages (PM) and antibacterial function of these ceils in nonphlogogenie and phlogogenic responses. MATERIALS AND METHODS Suspension of Staphylococcus aureus was injected intraperitoneally in outbred albino mice in dosesDepartment of Pathological Anatomy', Department of Pathological Phy~ology, Russian University of Peoples Frimadship, Moscow 25x 106 (series I) and 25x l0 s microbial bodies (series II). The doses were chosen on the basis of the literature data [4]. Experimental conditions were described previously [8]. Peritoneal lavage (PL) and blood were examined. They were centrifuged at 1500 rpm, the supernatant was discanted, and the pellet was used to prepare smears, which were examined by light microscopy. The smears were then fixed with 1% glutaraldehyde and viewed in an electron microscope. The intensity of staphylococcal invasion of the internal organs and blood was estimated by bacteriological methods. RESULTSIn both series of experiment mice developed punalent peritonitis, as evidenced by the appearance of the peritoneum. High mortality in the second series (about 99%) did not allow us to study the reaction of NL and PM at the late stages.Phagocytic activity of NL and PM from peritoneal lavage was observed 1 h after administration of St. aureus in both doses. These cells formed compact phagosomes containing one coccus. Phagocytized and nonphagocytized cocci were morphologically different. Free cocci were round, had a well outlined