Infectious pathology of pigs in the region is represented mainly bacterial diseases (52,8% of the total infectious diseases). However, the bacteria, as is usually secondary pathogens to cause clinical picture against viral disease. Among the latter occupies a special place reproductive and respiratory syndrome, characterized by obstetric and gynecological diseases in sows and respiratory problems in piglets. More and more reports have appeared in the world literature of the recent years, indicating the widespread distribution of the porcine reproductive and respiratory syndrome in North America and Europe. Currently, two types of virus are identified from pigs-the North American and European. PRRS is widespread also in Ukraine. The incidence in individual farms may exceed 55%, while the mortality observed in 2-6-month-old pigs varies from 1 to 25%. A serious decline in the growth and development of piglets is present. Viral diseases are one of the main causes of reproductive disorders in sows. The reproductive and respiratory syndrome (PRRS) is one of such diseases. That is why it is important to quickly and reliably determine the cause of the diseases in animals. A successful prevention of viral infections, which forms the basis of etiology of reproductive disorders, is dependent on an in-depth study of the nature of the viruses, antigenic and biological properties of their components, mechanisms of their relationship with cells, pathogenesis, factors of specific and nonspecific immunity as well as possible associations. The purpose of our research was a detailed study of the epizootic situation regarding the reproductive and respiratory syndrome of pigs in farms of different forms of ownership in the Chernukhinsky district of the Poltava region. In order to determine the role and place of PRRS in the infectious pathology of pigs in the conditions of the Chernukhinsky district of the Poltava region, we have examined the nozological profile, density, geographical distribution and annual dynamics of infectious diseases in

Porcine enzootic pneumonia is a multifactorial disease, it leads to large and significant economic losses in pig farms and it remains a relevant veterinary problem. The involvement of new etiological agents in the association of pathogens and the constantly changing conditions on the farms lead to the fact that previously developed control schemes and methods of disease lost their effectiveness. Therefore, the identification of all the infectious agents that make up the association, as well as the concomitant factors that led to the occurrence of the disease in each case is the only correct way to control measures.When we studied the situation in pig farms in the south and in the central region of Ukraine, we found that mycoplasmas were the main etiological agent for Porcine enzootic pneumonia. We noted that mycoplasmosis as a monoinfection was recorded only in 9-12 % of cases. In most outbreaks Mycoplasmas were only members of associations along with Porcine reproductive and respiratory syndrome infection (6%), Type 2 Circovirus infection (6%), enterbacterias (19%) and pasterellas (59%).The course of the disease was also aggravated by the stress of early weaning of piglets and violations of the conditions of feeding and animal welfare.The most difficult enzootic pneumonia occurred in those farms where mycoplasmosis and pasteurellosis were exacerbated by the presence of pathogens of actinobacillus pleuropneumonia. In such farms the average daily weight gain decreased in piglets by a third and feed consumption increased to 28 %.Piglets on affected farms were ill with obvious respiratory clinical signs. And 82% of dead or killed piglets had signs of fibrinous pleuropneumonia or catarrhal pneumonia.In those farms where we recorded associated respiratory infections, we also found reproductive-neonatal infections of pigs.In such farms treatment and prophylactic measures using traditional antimicrobial special remedies and monovaccines, did not give the expected effect.We proposed the use of autovaccines for associated enzootic pneumonia.

This abstract is devoted to development of method for rapid detection of the DNA-containing viral pathogens of pig in clinical materials using conventional multiplex PCR platform