The paper is devoted to the investigation of antimicrobial activity of polymer nanocomposites of both low-density polyethylene and nonwoven polymeric material (a mixture of woven and polyester fibers) that had been impregnated by Cu nanoparticles. The microorganisms were grown according to generally accepted microbiological rules and on media recommended for each bacteria family. Formation of biofilms of microorganisms was studied on the surface of microtiter plates for enzyme-linked immunosorbent assay. After incubation of microtiter plates, the culture medium was removed from the wells. The wells were rinsed five times with sterile distilled water. The plates were air dried for 45 minutes and each well was stained with 1% crystal violet solution in water for 45 min. After staining, the plates were washed with sterile distilled water five times. The quantitative analysis of biofilm production was done by adding 95% ethanol for discoloration of the wells. The research shows the antibacterial activity of Cu nanoparticles on planktonic forms of the investigated microorganisms, which prevented the formation of dense biofilms. With the use of low-density polyethylene impregnated by Cu the ability to form biofilms by planktonic cells of the referent strains of microorganisms was detected to decrease by 1.7 (Escherichia coli), 12.3 (Klebsiella pneumonia) times in the studied strains and with the use of nonwoven polymeric material treated by Cu nanoparticles, the ability to form biofilms decreased by 1.8 (Escherichia coli) – 21.8 (Klebsiella pneumonia) times in the studied strains. In subjecting the formed daily biofilms of referent strains of microorganisms to Cu nanoparticles, the destruction of biofilms of the studied strains of microorganism was observed as well as violation of the integrity of the biofilm monolayer and decrease of density index in comparison with control values. As a result, the obtained polymer nanocomposites can be recommended for preventive use in the fight against nosocomial infections. The practical relevance of this study lies in the possibility of reducing the incidence of purulent-inflammatory diseases and mycoses and, accordingly, reduction of the costs of treating these diseases.

The review article summarizes the date of literature and our own research on the creation of more effective and safe domestic combined drugs with a pronounced anti-inflammatory and analgesic effects. Untimely treatment of inflammatory processes often leads to long-term disability and even disability, so today there is an active search for new effective and safe domestic combination drugs with pronounced anti-inflammatory and analgesic effects. The constant search for innovative drugs causes a significant number of diseases, pathological conditions for which treatment with existing drugs is not effective or causes addiction and a large number of side effects. Because of, inflammatory processes can be accompanied by severe pain, spasms, increased convulsive activity of the brain, other disorders of the body, so non-narcotic analgesics and nonsteroidal anti-inflammatory drugs are combined with other drugs that can cause complementary effects or potentiate each other's effects. Caffeine is a useful adjuvant of nonsteroidal anti-inflammatory drugs of various chemical structures in terms of anti-inflammatory and analgesic effects. The review article presents a literature review of the current state of treatment of inflammation and pain, conducting research to expand the therapeutic capabilities of known pharmaceuticals, the creation of pharmaceutical compositions of nonsteroidal anti-inflammatory drugs with caffeine.

Determination of the effect of Ag nanoparticles impregnated in medical polyethylene products together with the LED radiation of the red and violet spectra on the ability of microorganisms to form biofilms and on daily biofilms.

Assessment of the biological effect of Cu nanoparticles impregnated in polyethylene medical devices combined with the effect of LED radiation of the red and violet spectra on the ability of microorganisms to form biofilms and on daily biofilms.