No abstract

The possible direct effect of gonadotrophin-releasing hormone (GnRH) and GnRH agonist (GnRH-A; buserelin) on basal and human chorionic gonadotrophin (HCG)-stimulated progesterone (P) and cyclic AMP (cAMP) production by cultured human luteal cells was examined. Luteal cells from the early or mid-luteal phase were incubated in long-term cultures. They responded to HCG stimulation with a 2- to 3-fold increase in P production and a 2-fold increase in cAMP production. The addition of GnRH (10(-7) and 10(-5) M) or GnRH-A (10(-7) and 10(-5) M) to the medium had no effect on either basal or HCG-stimulated secretion. These results indicate that both GnRH and GnRH-A have no direct effect on human luteal steroidogenesis in vitro.

No abstract

Abstract— A procedure has been developed which allows the isolation from rat brain cytosol of a soluble acidic protein, designated DNA‐110 protein, having two basic properties: selective affinity for single‐stranded DNA and immunological specificity to the nervous system. Only two major purification steps, DNA‐cellulose chromatography and affinity chromatography on immunoadsorbents are needed to give apparently pure protein. The purification steps of the DNA‐110 protein have been followed by immunological assay. DNA‐110 has a molecular weight of 68,000 and an isoelectric point of 5.9. It accounts for 1.95% of the total soluble protein and its concentration is 216 μg per g wet weight of rat brain. DNA‐110 is immunologically unrelated to other soluble acidic brain‐specific proteins and glycoproteins.