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SUMMARYDendritic cells (DC) represent a phenotypically heterogeneous population endowed with two important biological functions, immunity and tolerance. Here we report that the injection of splenic CD8 a + DC, derived from rats with experimental allergic encephalomyelitis (EAE), delayed the onset and suppressed the severity of EAE in Lewis rats. This was accompanied by the lack of magnetic resonance imaging (MRI) lesions in the brain and spinal cord and by reduced numbers of inflammatory cells within the central nervous system. Injection of CD8a+ DC inhibited T cell proliferation that may relate to increased interferon (IFN)-g and nitric oxide production. Although CD8 + CD28-suppressor T cells, apoptotic cells and co-stimulatory molecules were not altered, CD4+ T cells expressing interleukin (IL)-10 were augmented in rats receiving CD8 a + DC compared to rats receiving total DC or medium. These results demonstrate that rat splenic CD8 a + DC could provide a cellular basis for a novel, individualized immunotherapy using autologous DC as a complement to conventional therapy in diseases with an autoimmune background such as multiple sclerosis.

Impression cytology (from the Latin impressio — impact, indentation) is a minimally invasive method of cytological examination of the surface epithelium of the cornea, limbus and conjunctiva using various sorbing applicators for the collection of cellular material. The spectrum of diseases, in the diagnosis of which impression cytology is traditionally used, is mainly associated with pathological processes occurring in the conjunctiva. Such diseases as: Sjogren’s syndrome, dry eye syndrome, mucopolysaccharidosis, vitamin A deficiency, trachoma, squamous cell neoplasia of the ocular surface, etc. With the advent of the concept of limbal epithelial stem cell, new diagnostic possibilities of impression cytology in ophthalmology occurred.

The objective is to improve the results of treatment of locally advanced colorectal cancer in patients of older age groups due to the personalized approach to choosing surgical tactics with a comprehensive assessment of the functional operability and molecular biological properties of the tumor.Material and methods. A comparative evaluation of the results of surgical treatment of locally advanced colorectal cancer in patients of different age groups was conducted on representative clinical material. We studied the molecular profile of tumors, taking into account the age factor, and the clinical significance of specific markers in predicting the results of treatment.Results. The most significant indicators of functional operability of patients that must be considered when choosing a surgical tactic were identified. . The features of the immunophenotype of tumors, taking into account the age factor, were determined; and the prognostic significance of specific immunohistochemical indicators was estimated.Conclusion. The use of a personalized approach to choosing surgical tactics allows to achieve encouraging results of treatment in geriatric patients with locally advanced colorectal cancer.

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