Retention measurements of alkyl benzenes were carried out on the synthesized silica-based octyl bonded phases. The partition-displacement model was employed to analyze chromatographic properties of these phases. Namely, the methylene selectivity data were used to calculate the equilibrium sorption constant, the surface phase composition, and the sorption excess of water for each octyl phase. The differences in the surface phase composition of the phases studied were correlated with the structural and surface properties of the modified silica gels. 923

Introduction: Vanillin is one of the primary chemical components of the vanilla bean (Vanilla planifolia). An aphrodisiac is defined as any food or drug that arouses the sexual instinct, induces venereal desire and increases sexual pleasure and performance. Unpublished data claim that vanillin, taken under proper guidance, can help relieve problems of impotence, erectile dysfunction, frigidity, loss of libido and promotes arousal. Hence this study was conducted to study the potential aphrodisiac effects of vanillin in rats. Methods: Twenty four male rats were divided into four groups which received vehicle, vanillin 100 mg/kg/day, 200 mg/kg/day and 400 mg/kg/day orally, respectively. Female rats were brought to oestrous cycle by the sequential administration of estrogen (10 μg/100 g) and progesterone (0.5 mg/100 g) through intraperitoneal injections, 48 hours and 4 hours (respectively) prior to pairing with the male on day 1 to study the acute effects and on day10 to study the sub-chronic effects. Sexual behaviors were observed for three hours. Serum testosterone levels were estimated. The data was analyzed using one way ANOVA followed by posthoc tests. Results: There was a statistically significant increase in the mount frequency and intromission frequency compared to control following both acute and chronic treatment with vanillin (200 mg/kg). The mount latency, intromission latency, ejaculation latency and post-ejaculatory interval decreased in the vanillin treated groups especially at 200 mg/kg. There was no significant difference in the serum testosterone levels among the groups. Conclusion: Vanillin in the dose of 200 mg/kg demonstrated aphrodisiac properties in male wistar rats.

Background and Objectives:Vanillin is one of the primary chemical components of the extract of the vanilla bean. Vanillin has been claimed to possess various benefi cial effects like anti-mutagenic, anti-nociceptive, anti-invasive and metastasis inhibiting potential by suppressing enzymatic activity of matrix metalloproteinase-9. However, literature research revealed no scientifi c data on its anxiolytic activity. Hence, this study was designed to evaluate the anxiolytic activity of vanillin in wistar albino rats. Materials and Methods: The rats were divided into fi ve groups (n = 6). Vanillin administered at the dose of 10,100,200 mg/kg/day, orally was compared with the standard drug Diazepam (1.0 mg/kg/day, oral) fed for the latter 10 days. The two pharmacologically validated models, elevated plus maze and bright and dark arena were used. The data presented was analyzed using Kruskal Wallis followed by Mann-Whitney Test. P <0.05 was considered as statistically signifi cant. Result: Vanillin signifi cantly reduced the time spent in closed arm, increased the entries into open arm both in chronic and acute model of elevated plus maze (P < 0.05) in all three doses (10,100,200 mg/kg) used. Time spent in open arm, percentage ratio of open arm entries and number of rears in open arm also increased. Maximum effect was seen with 100 mg/kg. In bright and dark arena test there was an increase in number of entries, time spent and rears in bright chamber both in acute and chronic study at all doses (P < 0.05). Conclusion: The present study demonstrates the anxiolytic activity of vanillin in wistar albino rats.