Combination of photosensitizers (PS) with nanotechnology can improve the therapeutic efficiency of clinical Photodynamic Therapy (PDT) by converting visible light reactive PSs into Near-Infrared (NIR) light responsive molecules using Harmonic Nanoparticles (HNP). To test the PDT efficiency of HNP-PS conjugates, pathogenic S. aureus cell cultures were treated with perovskite (Barium Titanate) Second Harmonic Generation (SHG) nanoparticles conjugated to photosensitizers (PS) (we compared both FDA approved Protoporphyrin IX and Curcumin) and subjected to a femtosecond pulsed Near-Infrared (NIR) laser (800 nm, 232–228 mW, 12–15 fs pulse width at repetition rate of 76.9 MHz) for 10 minutes each. NIR PDT using Barium Titanate (BT) conjugated with Protoporphyrin IX as HNP-PS conjugate reduced the viability of S. aureus cells by 77.3 ± 9.7% while BT conjugated with Curcumin did not elicit any significant effect. Conventional PSs reactive only to visible spectrum light coupled with SHG nanoparticles enables the use of higher tissue penetrating NIR light to generate an efficient photodynamic effect, thereby overcoming low light penetration and tissue specificity of conventional visible light PDT treatments.

We present a numerical platform for 3D imaging and general analysis of multidimensional complex THz fields. A special 3D visualization is obtained by converting electromagnetic (EM) radiation to a light field via the Wigner distribution function, which is known for discovering (revealing) hidden details. This allows for 3D diagnostics using the simple techniques of geometrical optics, which significantly facilitates the whole analysis. This simulation was applied to a complex field composed of complex beams emitted as ultra-narrow femtosecond pulses. A method was developed for the generation of phase–amplitude and spectral characteristics of complex multimode radiation in a free-electron laser (FEL) operating under various parameters. The tool was successful at diagnosing an early design of the transmission line (TL) of an innovative accelerator at the Schlesinger Family Center for Compact Accelerators, Radiation Sources, and Applications.