Investigation of accumulation of different compounds in above-ground part of these plants an important aspect for evaluation of perspective of use. The aim of this study was to compare the peculiarities of the biochemical composition of Crambe species dynamically. Plant material collected from the experimental collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine. It was studied above-ground parts of C. cordifolia Steven, C. koktebelica (Junge) N. Busch, C. maritima L., C. steveniana Rupr. At the spring vegetation, budding stage, flowering, and fruitage. Following biochemical parameters was studied: dry matter by drying to consist weight at the 105 °C; content of sugars by Bertrand's method using of glucose scale; ascorbic acids with 2.6-dichlorophenolindophenol, tannins with indigo carmine discoloration, organic acids by sodium hydroxide titration with phenolphthalein; carotene with gasoline galosh spectrophotometrically; ash in muffle over. The dry matter during vegetation was from 9.76 (C. cordifolia, budding) to 22.54 (C. maritima at the fruitage) %, total content of sugars from 6.54 (C. maritime at the fruitage) to 33.18 (C. cordifolia at the budding) %, ascorbic acid from 139.85 (C. maritima at the spring vegetation) to 987.02 (C. maritime at the budding) mg%, b-carotene from 0.39 (C. maritima at the budding) to 1.82 (C. cordifolia at the fruitage) mg%, tannins from 1.28 (C. cordifolia at the fruitage) to 7.47 (C. steveniana at the budding) %, content of organic acids from 3.12 (C. steveniana at the fruitage) to 6.28 (C. koktebelica at the spring vegetation) %, ash from 6.11 (C. maritima at the spring vegetation) to 14.61 (C. cordifolia at the flowering) %. Correlation analysis showed that between the content of tannins and organic acids existed positive moderate correlation (r = 0.52). Between other parameters found the weak, very weak positive or negative correlation. Thus, comparing biochemical analyze identified that raw of four species of Crambe is a valuable source of nutrients during vegetation. The high content of sugars, ascorbic acid, and carotene can recommend these plants as forage crops. Also, raw of these plants can be recommended for farther pharmacological investigations.
The goal of this paper is to demonstrate a potential of energetic value of seeds of oil plants collected in the M.M. Gryshko National Botanical Garden of the NAS of Ukraine. In the present study lipid and caloric content of cultivars and varieties of Brassica campestris D.C., Brassica junceae (L.) Czern., Brassica napus D.C., Brassica rapa subsp. rapifera Metzger, Camelina sativa (L.) Crantz and Sinapis alba L. are described. Studing of seeds of 19 samples of investigated plants was carried out by method of extraction with petroleum ether equipped with Soxhlet extractor. Content of lipids was determined in range from 17.72% (Brassica campestris f. annua D.C., cv. Pioner) to 37.61% (Brassica napus f. annua D.C., cv.s. Yamal). Comparing of Brassica campestris plants showed that winter varieties had higher level of lipids in seeds and was in range from 28.35 to 34.48%. In the spring varieties the same parameter was in range from 17.72 to 29.00%. Identification of energetic value was made by method of combustion in the oxygen and was determined in range from 5,039.33 Kcal/kg (Brassica campestris f. annua) to 6,108.00 Kcal/kg (Brassica napus f. annua D.C., cv.s. Yamal). Content of calories in the seeds of winter varieties of Brassica campestris was from 5,530.40 to 6,045.67 Kcal/kg and in spring varieties -from 5,381.33 to 5,659.11 Kcal/kg.
Asteraceae Bercht. & J. Presl. is a large family of plants with a different direction of use, among which are medicine, decorative, dietary crops that widely have used in human life. Plant raw material of these plants is a source of biologically active compounds with numerous biological activities such as antioxidant, antimicrobial, anti-inflammatory, etc. This study was aimed to evaluate the antioxidant potential of selected plants from Asteraceae: Bidens ferulifolia (Jacq.) Sweet, Echinacea purpurea (L.) Moench, Rhaponticum carthamoides (Willd.) Iljin, Silphium asteriscus L., S. lacinatum L., S. perfoliatum L., S. trifoliatum L. Raw collected from the collection of M.M. Gryshko National Botanical Garden of the NAS of Ukraine (NBG) in the stage of budding and flowering. Determination of the antioxidant activity of investigated plants conducted by DPPH free radical scavenging activity. In this case, 1 g of dried and milled plant raw extracted in methanol and water in volume 25 ml and 100 µl of filtrate mixed with 3.9 ml of a radical solution. The optical density of the plant extracts measured using the spectrophotometer Unico 2800 (Russia) at the wavelength of 515 nm. Results expressed in % of inhibition. The DPPH free radical scavenging activity of methanol extracts of Bidens ferulifolia was 72.27-86.69%, E. purpurea of 31.19-75.92%, Rh. carthamoides of 56.31-73.14% and Silphium spp. from 34.86 to 92.51%. This parameter in water extracts was for Bidens ferulifolia 14.68-62.07%, E. purpurea of 45.47-68.90%, Rh. carthamoides of 52.61-79.05%, Silphium spp. from 14.88 to 93.47%. Thus, a study of the inhibition ability of different extracts of selected Asteraceae plants from NBG demonstrated the antioxidant potential of investigated plants than can be used for further study. Also, raw of these plants can be recommended for farther pharmacological investigations and as useful forage plants.
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