Background Wolbachia bacteria are widely distributed throughout terrestrial arthropod species. These bacteria can manipulate reproduction and influence the vector competence of their hosts. Recently, Wolbachia have been integrated into vector control programmes for mosquito management. A number of supergroups and strains exist for Wolbachia, and they have yet to be characterized for many mosquito species. In this study, we examined Wolbachia prevalence and their phylogenetic relationship to other Wolbachia, using mosquitoes collected in Merced County in the Central Valley of California. Methods Adult mosquitoes were collected from 85 sites in Merced County, California in 2017 and 2018. Traditional and quantitative PCR were used to investigate the presence or absence and the density of Wolbachia, using Wolbachia-specific 16S rRNA and Wolbachia-surface protein (wsp) genes. The supergroup of Wolbachia was determined, and Multilocus Sequence Typing (MLST) by sequencing five housekeeping genes (coxA, gatB, ftsZ, hcpA and fbpA) was also used to determine Wolbachia supergroup as well as strain. Results Over 7100 mosquitoes of 12 species were collected: Aedesmelanimon, Ae. nigromaculis, Ae. vexans, Ae. aegypti, Culex pipiens, Cx. stigmatosoma, Cx. tarsalis, Anopheles franciscanus, An. freeborni, An. punctipennis, Culiseta incidens and Cs. inornata. Eight showed evidence of Wolbachia. To our knowledge, this study is the first to report detection of Wolbachia in five of these species (Ae. melanimon, Cx. stigmatosoma, Cx. tarsalis, Cs. incidens and Cs. inornata). Culex pipiens and Cx. stigmatosoma had a high frequency and density of Wolbachia infection, which grouped into supergroup B; Cs. inornata clustered with supergroup A. MLST comparisons identified Cx. pipiens and Cx. stigmatosoma as wPip strain type 9 supergroup B. Six species had moderate to low (< 14%) frequencies of Wolbachia. Four species were negative, Ae. nigromaculis, An. franciscanus, An. freeborni and Ae. aegypti. Conclusions New records of Wolbachia detection were found in mosquitoes from Merced County, California. Culex stigmatosoma and Cs. inornata were new records for Wolbachia supergroup B and A, respectively. Other species with Wolbachia occurred with low frequency and low density. Detection of Wolbachia in mosquitoes can be used to inform potential vector control applications. Future study of Wolbachia within Cx. stigmatosoma and Cs. inornata in California and through the range of these species could further explore Wolbachia infection in these two species.

To evaluate methodologically (PRISMA) and report (AMSTAR-2) quality of systematic reviews regarding platelet rich plasma (PRP) as a method of tissue regeneration in dental procedures. in English or Spanish. The advanced search was performed using Boolean terms AND and OR. The quality of the SRs was evaluated with the PRISMA guidelines, and the methodological quality was evaluated with the checklist of the AMSTAR-2 tool. Result: Seventeen SRs were selected. From the selected articles, 11 were cataloged as of Very good quality, three as Good and three as Moderate. Regarding the methodological quality, two articles were classified as Critically Low, one as Low, 10 Moderate and four as High quality. Conclusion: Most SRs are of high quality regarding their report and methodology, and provide an accurate summary of the results of the primary studies available. However, there is a lower percentage of RS whose methodological and drafting deficiencies make their results and conclusions unreliable, and therefore, their clinical applicability is questionable, and offer a wrong clinical vision, which is not the best and safest therapeutic measure for patients and may compromise their well-being.Resumen: Evaluar la calidad metodológica (PRISMA) y del reporte (AMSTAR-2) de las revisiones sistemáticas en relación al plasma rico en plaquetas (PRP) como método de regeneración tisular en odontología. Material y Metodos: Se realizó una búsqueda de revisiones sistemáticas (RS) de PRP en las bases de datos PUBMED, EBSCO, The Cochrane Library, Web of Science, Lilacs, Epistemonikos, y complementada mediante revisión manual de bibliografía de los artículos seleccionados. Abarcando artículos publicados desde Enero de 2008 hasta Agosto de 2018, en idioma inglés o español. La búsqueda avanzada se realizó utilizando términos booleanos AND y OR. Las RS fueron evaluadas con pauta PRISMA la calidad del reporte, y con checklist de la herramienta AMSTAR-2 la calidad metodológica. Resultados: Fueron seleccionadas 17 RS. Los artículos seleccionados fueron catalogados con calidad del reporte Muy bueno 11 artículos, 3 como Buenos y 3 como Regulares. En relación a la calidad metodológica, 2 artículos fueron catalogados como Críticamente baja, 1 Baja, 10 Moderada y 4 alta calidad. Conclusión: La mayoría de las RS cuentan con calidad en su reporte y en su metodología, y proveen un resumen preciso de los resultados de los estudios primarios disponibles. Sin embargo, hay un porcentaje menor de RS cuyas deficiencias metodológicas y de redacción hacen no fidedignos sus resultados y conclusiones y, por tanto, su aplicabilidad clínica es cuestionable y puede formar un juicio clínico errado que no sea la mejor y más segura medida terapéutica para el paciente y comprometer su bienestar. Palabras Clave: Odontología; plasma rico en plaquetas; revisión sistemática; calidad; informe de investigación; lista de verificación.

Este estudio indaga sobre los discursos de masculinidad a partir de experiencias cotidianas de representantes de asociaciones escuelas, pues persisten retos en la deconstrucción de una masculinidad hegemónica, dado que 1 de cada 2 estudiantes ha sufrido acoso sexual (Universidad de Cuenca, 2022), y un 83% de estudiantes hombres son indiferentes a la masculinidad tradicional (Orellana y Yanza, 2018). Por ello, esta investigación cualitativa buscó describir, con enfoque psicosocial y de género, discursos de masculinidad y sus tensiones, con un grupo de 14 hombres cisgénero. Los participantes mostraron discursos de masculinidad tradicionales y alternativos en procesos de conformidad social y construcción de lo masculino. Hubo un discurso hegemónico con estrategias de nominación sobre géneros y legitimación patriarcal, con una versión del feminismo contra ellos. También, hubo discursos minoritarios sobre las tensiones por la restricción emocional, violencia y estereotipos masculinos.

The names of feminism are acts of xenoglossia that disseminate other speech and writing regimes susceptible to mismatch the regularly known, legible and legitimate formats. They are alchemical acts that invent modes of existence, strange languages, ways of doing (micro) politics, with the waste of the heteropatriarchal semiotic machine that governs words and lives and with the detritus of the institutionalization and normalization of feminism. Names like micro-creations of fictions that assemble new connections of intensities, disseminate unpublished meanings of the sex-political resistance from the south, with their unexpected marks, their harsh textures, their extravagant tones. In these errancias by the names, delving into the remnants of hypervisibility, the body becomes sensitive and political platform of feminist subversion and from five febrile alchemies of the body as techniques of corporal knowledge, political skills, affective constellations and erotic micropowers of feminist doing, an excremental feminism is composed.