One of the new promising candidate genes defining productive qualities of sheep is MEF2B. Protein from the MEF2 group encoded by it affects the production of myostatin and the expression of the genes responsible for the growth of skeletal muscle fibers. Thus, the knowledge of the MEF2B gene structure is important for genomic selection. We have studied the structure of the MEF2B gene at sheep of Severokavkazskaya breed bred in Russia. To detect alleles we use NimbleGen sequencing technology by Roche (USA). As a result, it was revealed 14 single nucleotide polymorphisms (SNP) at the given breed. The discovered SNPare located in not coding areas. From them 7 polymorphisms are in the area of 5? upstream gene in loci: c.-1713, c.-1319, c.-839, c.-321, c.-246, c.-161, c.-3; 6 polymorphisms are in introns, loci: c.55-51, c.258+312, c.258+380, c.259-52, c.452+95, c.452+103, 1 SNP is in 3? downstream gene, c.

Targeting is a phenomenon in which the distribution of a drug in the body occurs in such a way that the main part of it interacts with the target tissue at the cellular or subcellular level to achieve the desired pharmacological effect on the selected site without undesirable interactions in other organs. This can be achieved using a drug delivery system such as niosomes, which are non-ionic vesicles of surfactants obtained by hydrating synthetic non-ionic surfactants with the inclusion of cholesterol. They are vestibular systems similar to liposomes that can be used as carriers of amphiphilic or lipophilic drugs. Niosomes are a promising drug delivery tool, and it has been widely evaluated as a possibility of controlled release and targeted delivery of the active substance for the treatment of cancer, autoimmune diseases, viral and other infectious diseases. It can be assumed that encapsulation of the drug in the vesicular system prolongs its presence in the systemic circulation and increases the possibility of penetration into the target tissue, possibly reducing toxicity if selective absorption can be achieved.

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The work on the inhalation toxicity of the ozone-air mixture was carried out in two stages. At the first stage, acute inhalation toxicity was studied and the class of toxicity was determined, at the second stage, subchronic inhalation toxicity was determined. The object of the study was an ozone-air mixture. A portable standalone ozone generation device developed by us was used as a source of the mixture. A total of 58 outbred mature white Wistar rats of both sexes were used in the study, where 18 rats participated in the first stage, and 40 rats in the second. The animals were placed in an inhalation chamber. The procedure for testing acute inhalation toxicity was carried out according to the standard method at concentrations of ozone-air mixture of 100, 500, 2500 ppm and an exposure of 4 hours. As a result, the fatal outcome was observed only in 3 groups (2500 ppm), 3 out of 6 individuals were killed, including 2 males and 1 female. In fallen animals, death occurred as a result of respiratory arrest. The surviving animals were monitored for 14 days after the test. During the treatment of animals with ozone-air mixture, a gradual inhibition of the respiratory system was observed, followed by the development of pulmonary edema and respiratory failure. Based on the data obtained, the ozone-air mixture under study was assigned a hazard class 3. In the study of subchronic inhalation toxicity of oats, concentrations of 250, 125, and 50 ppm were studied. The experiment was carried out, according to the standards for determining the subchronic inhalation toxicity of substances, for 90 days. During the experiment, body weight, feed consumption, behavioral activity were recorded, and blood hematological and biochemical parameters were determined. As a result of the study of subchronic inhalation toxicity of the ozone-air mixture, no signs of intoxication were detected in rats, and there were no death cases.

The subchronic inhalation toxicity of the ozone-air mixture obtained on a portable ozone generation device (ozonizer) of its own design was studied.  The work investigated the concentration of ozone-air mixture of 1000, 3000 and 5000 ppm.  The experiment was carried out, according to the standards for determining subchronic inhalation toxicity of substances, for 90 days, on rats of the Wistar strain.  Throughout the experiment, body weight, feed intake were recorded, behavioral activity was evaluated, hematological and biochemical blood parameters were determined.  As a result of the study of subchronic inhalation toxicity of the ozone-air mixture, no signs of intoxication were detected in rats, there was no case.