Objectives
The present study assessed the sensitivity and biosafety of the FTA Elute Card® (FTA) (GE Healthcare) as an alternative preservation method of gross lesions for bovine tuberculosis diagnosis, that can endure large distances and high environmental temperatures, which are found in the Brazilian territory.
Results
The FTA card was compared to the freezing and sodium borate solution methods to preserve 134 gross lesions samples from slaughterhouses located in three Brazilian states. The calculated value for the sensitivity of sodium borate solution (SBS) stored for 30 days was higher than those estimated for the methods of freezing and SBS stored for 60 days, when the PCR and isolation results were interpreted in parallel (0.55). The FTA cards presented the lowest diagnostic sensitivity and did not inactivate all mycobacteria. Given the rush of a slaughterhouse routine, the quantity of lesions with different pathological stages cannot be measured on each FTA card. Thus, as a preservation method of gross tuberculosis lesions, the FTA cards can present infection risks for people who have direct or indirect contact with them.
Este estudo foi realizado para identificar os fatores relacionadoscom o consumo de pescado no Mercado Municipal Paulistano(MMP). Para esse fim, foram entrevistadas 100 pessoas: 65 mulherese 35 homens. Os entrevistados responderam a um questionário,preparado com perguntas abertas e fechadas sobre ascaracterísticas gerais do consumidor e o hábito de consumo depescado. Os dados resultantes da pesquisa foram tabulados emum software de edição de planilhas a partir das quais foramconstruídos gráficos e tabelas. A maioria dos entrevistados eramulher e possuía residência e origem no município de São Paulo.Metade dos entrevistados relatou ir raramente ao mercado e osque iam com frequência indicaram que a variedade e a qualidadedo produto são fatores importantes no MMP. Observa-seque os fatores que mais dificultam o consumo de pescado sãoo preço e a falta de hábito de consumo do produto pelos entrevistados.Conclui-se que o Mercado Municipal é uma local commuitas opções e com produtos de boa qualidade de pescado,mas o alto preço e a falta de hábito do consumo são fatores querestringem a frequência de consumo.
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