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Application of enzymes for the removal of environmentally hazardous synthetic dyes from waste water has been considered eco-friendly and economic as compared with nonenzymatic techniques. In the present study, response surface methodology has been applied to decolorize and detoxify a recalcitrant and toxic anthraquinone dye Remazol Brilliant Blue R (RBBR) using Coriolopsis caperata DN laccase. Optimum concentrations of laccase, pH, and temperature for decolorization of RBBR dye (100 to 500 ppm) were 0.5 U ml −1 , 3.5, and 40°C, respectively. Result of RSMshowed that optimum value of tested variables for maximum dye decolorization was 1 U ml −1 enzyme, 1000 ppm dye, and 60 min. Result of kinetic study showed that the K m , V max , K cat , and K cat /K m values for RBBR decolorization were 1.06 mM, 0.226 mM U −1 min

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