The quantitation of cells bearing CD3, CD4, CD8, and B cell phenotypic markers, as well as an estimation of serum immunoglobulin (Ig)G, IgA, and IgM, was carried out in a group of 39 glioma patients with different grades of malignancy. The findings were compared with those obtained from 21 normal healthy control subjects. The analysis revealed a significant decrease both in the absolute numbers and in the percentages of circulating CD3+ (p less than 0.001) and CD4+ (p less than 0.001) cells, while the CD8+ and Pan B+ cells remained within the normal range irrespective of the type and grade of tumor. The CD4+:CD8+ ratio was significantly decreased in all categories of patients. The CD4 lymphopenia was also evident in 10 patients who had no history of previous immunosuppressive drug therapy (steroids and anticonvulsants) until the commencement of the study. The Ig levels were within the normal range in patients with malignant astrocytoma and glioblastoma multiforme, whereas a three- and fourfold increase in the IgM level was observed in patients with astrocytoma. It is suggested that T cell lymphopenia in glioma patients could mainly be due to a selective depletion of CD4+ cells and that it occurs principally as a reaction to the tumor.

The paper describes the results of in-vitro experiments to determine the contact areas in the elbow joint during different anatomical positions. The casting technique, using wax as a casting material, was used in this study. The shape and size of the contact areas change, in different elbow positions ranging from full extension to full flexion. The joint stability was preserved during the experiments. In full extension the area of contact was observed on the lower-medial aspect of the ulna while in other postures the pressure areas were found as a strip extending from posterolateral to anteromedial. The radio-capitulum joint also revealed contact during flexion under no externally applied loads.

Golgi-stained sections of the human cerebellar dentate nucleus (DN) at various gestational ages were examined to study the dendritic proliferation and maturation of the DN neurons. Bipolar cells were seen at 14–15 weeks. By 19–20 weeks, three cell types were identifiable: bipolar, hemisphereic and pyriform. The cells of the dorsomedial region matured earlier than those of the ventrolateral region. In addition to the above cell types, multipolar and nuclear boundary cells were observable at 24–25 weeks. The five cell types persisted during subsequent development. At no stage of development was any neuronal organizational pattern apparent. A remarkable dendritic proliferation occurred at 27–28 weeks. Spines and filiform processes were seen at 34 weeks of intrauterine life.

Undernutrition of the newborn rats, produced during the first 3 weeks by increasing the litter size and restricting the mother's diet, resulted in reduction of the body and brain weights of the experimental animals. One group of undernourished animals showed especially severe reduction of body and cerebellar weights. These animals, on the 10th postnatal day, had an immature cerebellar cortex corresponding to that of the 7th day postnatal control animals. The external granular layer persisted in the cerebellar cortex of the underweight animals until the 23rd day, while it disappeared by 20th day in the control animals. Mitotic activity was evident until the 21st postnatal day in these animals while it stopped in the normal animal by 16th postnatal day. There was no marked difference in the fine structure of the various cell types in the control and undernourished animals. Midsagittal tracings of the cerebellar cortex showed a reduced surface area in the undernourished animals, while the thickness of the external granular layer and molecular layer did not show any significant difference when compared to that of the control animals, thus showing a reduction in total cell number, but not per unit area. The normal morphological appearance of the cerebellar cortex in the underfed animals of higher weight probably indicates that these animals are adequately nourished in spite of the reduction in weight when compared to the control animals, which probably are overfed.

A qualitative and quantitative ultrastructural study has been carried out on lateral geniculate nuclei (LGN) of 21 human fetuses ranging in gestational age from 13-14 to 34-35 weeks. At the early age period of 13-15 weeks, LGN is characterized by immature cells with indented nuclei possessing multiple nucleoli and by a sparse neuropil. During the subsequent age periods studied progressive maturational changes lead to neurons having round nuclei with a single nucleolus and well-developed cytoplasmic organelles as well as to an elaborate neuropil. Synaptic contacts which are seen for the first time at the age of 13-14 weeks are of retinogeniculate type. They show features of immature synapses and are located mainly on the juxtasomatic parts of dendrites. With increasing gestational age, the synapses increase in size, maturity, types and complexity; an acquisition of complex synaptic arrangement (triad) occurs by 20-21 weeks. Excitatory synapses appear earlier than do the inhibitory ones. Formation of retinogeniculate contacts precedes that of the corticogeniculate type. The synaptic density and total synapse number show a progressive increase with increasing gestational age. The age period of 15-20 weeks of gestation is marked by presence of organelles suggestive of a high rate of metabolic activity, significant increase in synaptogenesis, presence of transient contacts on soma and large number of free postsynaptic membrane densities (PSD). The period thereby represents a critical period in the development of synapses in LGN. The numerical values obtained by by the age of 34-35 weeks are still low as compared to the values reported for other areas of brain.