No abstract

No abstract

No abstract

The synthesis and luminescence properties are reported for 20 different chelates composed of 2,2':6',2"-terpyridine as the energy-absorbing and donating group, Eu"' and Tb"' as the emitting ions, methylenenitrilo(acetic acids) as the stable chelate-forming moieties, and isothiocyanato or (4,6-dichloro-1,3,5-triazin-2-yl)amino groups as the activated moieties for coupling to biomolecules.Introduction. -Time-resolved fluorometry combined with long-lifetime emitting lanthanide chelate labels provides an excellent way of creating highly sensitive label technologies for bioaffinity assays [ 11. A technology based on dissociative fluorescence enhancement [2], Devia@, has gained wide applications in the field of clinical diagnostics in immunoassays [3] and recently also in DNA hybridization assays [4]. In spite of the high sensitivity obtained, the Devia-type of technology is not suited for all applications, such as fluorescence imaging, immunohistochemistry, or in situ hybridization, because after ion dissociation it does not produce spatial information. To use luminescent lanthanide chelates also in in situ assays, new chelate labels need to be developed combining