Zebrafish is being used as a model for preclinical studies because of its unique innate physiological and behavioral aspects that almost correlate to the mammals including human. Many behavioral models have been developed based on visual stimuli, appetite conditioning, colour preference ability of zebrafish. In the present study, we used two different colors (Red and Green) which are the equal preference of zebrafish to avoid the bias and food was placed in one of the colored short arms (Green) only during the training sessions. In the test session, food was not placed in the green arm and animals were evaluated for its learning and memory in finding out the food. For evaluating the newly designed T-Maze, we employed two drugs, Scopolamine, that induce memory impairment and Rivastigmine that ameliorates the effects of scopolamine. In the test session, animals treated only with scopolamine spent less time in the green arm and found to have more number of total entries unlike the animals pre-treated with Rivastigmine one hour before scopolamine treatment. Results showed that Rivastigmine reduced the memory impairment effects of scopolamine. The findings from the present study suggest that the newly designed color bias avoided and appetite conditioning T-Maze was found to be useful for evaluating the memory enhancing drugs effectively.

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