Eleven novel pregnane glycosides, 2-7 and 9-13, of which four, i.e., 10-13, comprised a new pregnane-type genin exhibiting a hydroxymethylene instead of a Me group at C(19), and the known pregnane glycoside stalagmoside V (8) were isolated from whole plants of Caralluma adscendens var. fimbriata, a native Indian succulent plant. Their structures were elucidated by extensive 2D-NMR spectroscopic studies.

Effects of refrigeration (5 ± I'C) of newly hatchet! silkworm larvae on survival rate, growth and egg production were studied in two multivoltine races, Pure Mysore and Hosa Mysore. When refrigerated for 10 days, moth emergence (males: 83·3% vs 89·4% and females: 92% vs 90'2%) and fecundity (465'5 vs 458'3) did not differ significantly from the control. Reductions in cocoon weight by 4% (1,323 g vs 1·271 g) and 10·7% (1'323 g vs 1·181 g) were caused by 5 and 10 days of refrigeration. Marked increase in early stage (I and II instar), loss of larvae (12'9% vs 48,8%), decrease in effective rate of rearing (77'1% vs 33'5%), pupation rate (92,4% vs 65%) and reproductive rate (137,7 vs 36'9) were observed, when refrigerated for 10 days. Results on reproductive rate indicated one day's refrigeration as safe period in Hosa Mysore but not in Pure Mysore. In December season. both races showed higher tolerance and possibility of safe refrigeration upto 2 days.