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Bio-glass® and hydroxyapatite (Ca10 (PO4)6(OH) 2, HA) has been widely used as a bone replacement material in restorative dental and orthopedic implants. In order to analyze in vitro bioactivity bio-composite before and after exposed to simulated body fluid (SBF) solution for different time periods were investigated by fourier transform infrared (FTIR) reflectance spectrometer with measuring the pH and concentration of silicon, sodium, calcium, phosphorus and manganese ions in SBF solution. The prepared bio-composites were assessed by XRD, FTIR, mechanical properties. FTIR confirmed the presence of a rich bone like apatite layer post-immersion on the composite surface. It has been found that the new BG/HA bio-composite materials have high bioactivity properties. These bio-composite materials are promising for medical applications such as bone substitutes.