Key message In the QTL analysis of multi-parent populations, the inclusion of QTLs with various types of effects can lead to a better description of the phenotypic variation and increased power. AbstractFor the type of QTL effect in QTL models for multi-parent populations (MPPs), various options exist to define them with respect to their origin. They can be modelled as referring to close parental lines or to further away ancestral founder lines. QTL models for MPPs can also be characterized by the homo- or heterogeneity of variance for polygenic effects. The most suitable model for the origin of the QTL effect and the homo- or heterogeneity of polygenic effects may be a function of the genetic distance distribution between the parents of MPPs. We investigated the statistical properties of various QTL detection models for MPPs taking into account the genetic distances between the parents of the MPP. We evaluated models with different assumptions about the QTL effect and the form of the residual term using cross validation. For the EU-NAM data, we showed that it can be useful to mix in the same model QTLs with different types of effects (parental, ancestral, or bi-allelic). The benefit of using cross-specific residual terms to handle the heterogeneity of variance was less obvious for this particular data set.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-017-2923-3) contains supplementary material, which is available to authorized users.

Key message Multi-parent populations multi-environment QTL experiments data should be analysed jointly to estimate the QTL effect variation within the population and between environments. Abstract Commonly, QTL detection in multi-parent populations (MPPs) data measured in multiple environments (ME) is done by analyzing genotypic values ‘averaged’ across environments. This method ignores the environment-specific QTL (QTLxE) effects. Running separate single environment analyses is a possibility to measure QTLxE effects, but those analyses do not model the genetic covariance due to the use of the same genotype in different environments. In this paper, we propose methods to analyse MPP-ME QTL experiments using simultaneously the data from several environments and modelling the genotypic covariance. Using data from the EU-NAM Flint population, we show that these methods estimate the QTLxE effects and that they can improve the quality of the QTL detection. Those methods also have a larger inference power. For example, they can be extended to integrate environmental indices like temperature or precipitation to better understand the mechanisms behind the QTLxE effects. Therefore, our methodology allows the exploitation of the full MPP-ME data potential: to estimate QTL effect variation (a) within the MPP between sub-populations due to different genetic backgrounds and (b) between environments. Electronic supplementary material The online version of this article (10.1007/s00122-020-03621-0) contains supplementary material, which is available to authorized users.

Multi-parent populations (MPPs) are important resources for studying plant genetic architecture and detecting quantitative trait loci (QTLs). In MPPs, the QTL effects can show various levels of allelic diversity, which is an important factor influencing the detection of QTLs. In MPPs, the allelic effects can be more or less specific. They can depend on an ancestor, a parent or the combination of parents in a cross. In this paper, we evaluated the effect of QTL allelic diversity on the QTL detection power in MPPs.We simulated: a) cross-specific QTLs; b) parental and ancestral QTLs; and c) bi-allelic QTLs. Inspired by a real application, we tested different MPP designs (diallel, chessboard, factorial, and NAM) derived from five or nine parents to explore the ability to sample genetic diversity and detect QTLs. Using a fixed total population size, the QTL detection power was larger in MPPs with fewer but larger crosses derived from a reduced number of parents. The use of a larger set of parents was useful to detect rare alleles with a large phenotypic effect. The benefit of using a larger set of parents was however conditioned on an increase of the total population size. We also determined empirical confidence intervals for QTL location to compare the resolution of different designs. For QTLs representing 6% of the phenotypic variation, using 1600 offspring individuals, we found 95% empirical confidence intervals of 50 and 26 cM for cross-specific and bi-allelic QTLs, respectively.MPPs derived from less parents with few but large crosses generally increased the QTL detection power. Using a larger set of parents to cover a wider genetic diversity can be useful to detect QTLs with a reduced minor allele frequency when the QTL effect is large and when the total population size is increased. 1The influence of QTL allelic diversity in MPPs due to a better control over the population structure [1]. Here, we focus on MPPs composed of bi-parental crosses without further intercrossing. This definition does not cover MPPs like the multi-parent advanced generation inter-cross (MAGIC) populations [2]. Different statistical procedures exist to detect QTLs in MPPs but generally those methods, like the one adapting models used in genome-wide association studies [3,4], do not model properly the diversity of allelic effects present in MPPs. Similarly, most of the MPPs simulation studies have not simulated the wide range of QTL allelic effects present in those populations. Therefore, we investigated the QTL detection power in MPPs using scenarios that accounted better for the MPP QTL allelic diversity. We also determined empirical confidence intervals for the detected QTLs, which is an essential information for marker assisted selection (MAS) [5]. To the extent of our knowledge, no article provides such information in MPPs composed of crosses. MPP designMany MPP designs have been evaluated through simulation studies [3,6,7,8]. The nested association mapping (NAM) design is a collection of crosses between a central parent and peripher...

The rapid development of phenotyping technologies over the last years gave the opportunity to study plant development over time. The treatment of the massive amount of data collected by high-throughput phenotyping (HTP) platforms is however an important challenge for the plant science community. An important issue is to accurately estimate, over time, the genotypic component of plant phenotype. In outdoor and field-based HTP platforms, phenotype measurements can be substantially affected by data-generation inaccuracies or failures, leading to erroneous or missing data. To solve that problem, we developed an analytical pipeline composed of three modules: detection of outliers, imputation of missing values, and mixed-model genotype adjusted means computation with spatial adjustment. The pipeline was tested on three different traits (3D leaf area, projected leaf area, and plant height), in two crops (chickpea, sorghum), measured during two seasons. Using real-data analyses and simulations, we showed that the sequential application of the three pipeline steps was particularly useful to estimate smooth genotype growth curves from raw data containing a large amount of noise, a situation that is potentially frequent in data generated on outdoor HTP platforms. The procedure we propose can handle up to 50% of missing values. It is also robust to data contamination rates between 20 and 30% of the data. The pipeline was further extended to model the genotype time series data. A change-point analysis allowed the determination of growth phases and the optimal timing where genotypic differences were the largest. The estimated genotypic values were used to cluster the genotypes during the optimal growth phase. Through a two-way analysis of variance (ANOVA), clusters were found to be consistently defined throughout the growth duration. Therefore, we could show, on a wide range of scenarios, that the pipeline facilitated efficient extraction of useful information from outdoor HTP platform data. High-quality plant growth time series data is also provided to support breeding decisions. The R code of the pipeline is available at https://github.com/ICRISAT-GEMS/SpaTemHTP.

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