Nowadays some efforts are being made to reduce obesity by using drugs that aim to reduce appetite of the drug users. However, the use of drugs still has harmful side effects. Endophytic fungi originating from the Dayak onion plant are thought to have secondary metabolites that have potential as anti-obesity. The aims of this study were to determine the compounds produced by endophytic fungi of Dayak onion and to determine the potential components as inhibitors of pancreatic lipase enzymes. Samples of Dayak onions were collected from Palangka Raya using purposive sampling method. Isolation of endophytic fungi was conducted using surface sterilization method and the isolates were grown on PDA. Isolates were characterized and fermented on PDB for 14 days. The selected isolate was analyzed by using GCMS then molecular docking was performed using Autodock Vina and PyRx. The research results showed that there were 7 isolates of endophytic fungi isolated from leaves and roots tissues of Dayak onion. GCMS analysis showed that EBD1 contains 38 compounds. Molecular docking analysis showed that 1,3-Di-O-Acetylpentopyranose, a test ligand, has the closest binding affinity value (-6.6 kcal/mol) to orlistat (control) (-6.8 kcal/mol). Thus, it has the potential as an inhibitor of pancreatic lipase enzyme protein.
Jamur makroskopis adalah makhluk hidup heterotrof yang sangat beragam dan kebanyakan adalah saprofit. Penelitian ini bertujuan untuk mengidentifikasi keanekaragaman jenis jamur makroskopis di kawasan hutan Universitas Palangka Raya. Sampel jamur dikoleksi pada bulan Mei hingga Juni 2021 menggunakan metode purposive sampling. Identifikasi jamur dilakukan berdasarkan karakter morfologi seperti bentuk tubuh buah, lamela, bentuk dan warna tudung jamur. Hasil penelitian diperolej ada 18 spesies jamur makroskopis Basidiomycota yang terdiri dari 9 suku dan 12 marga. Spesies yang berhasil diidentifikasi tersebut adalah Agrocybe sp., Auricularia sp.1, Auricularia sp.2,Collybia sp., Dacryopinax spathularia, Ganoderma sp.1, Ganoderma sp.2, Ganodermasp.3, Hygrocybe sp., Marasmiellus sp., Mycena sp.1, Mycenasp.2, Pycnoporus sanguineus, Schizophyllum commune, Trametes sp.1,Trametessp.2, Trametes sp.3, dan Tyromyces sp. Deskripsi masing-masing spesies dicantumkan.
Fusarium oxysporum merupakan penyebab penyakit layu dan banyak mematikan tanaman pisang. Cendawan ini diketahui mampu hidup dan bertahan sebagai endofit pada gulma dan menjadi sumber inokulum yang nantinya menyebabkan penyakit pada tanaman pisang. Penelitian ini dilakukan untuk mengisolasi F. oxysporum dari akar gulma yang tumbuh di sekitar pertanaman pisang di Bogor, Jawa Barat, kemudian menguji sifat patogenisitasnya. Identifikasi morfologi dilakukan dengan mengamati koloni, konidium, dan klamidosporanya. Uji patogenisitas isolat endofit dilakukan di laboratorium menggunakan bibit pisang var. Raja Bulu. Sebanyak 9 isolat F. oxysporum berhasil diisolasi dari gulma yang tumbuh di sekitar pertanaman pisang. Semua isolat menunjukkan ciri morfologi sebagai F. oxysporum, yaitu koloni berwarna ungu, makrokonidium berbentuk kano dengan tiga sekat false head pendek, mikrokonidium berbentuk silindris atau ginjal, dan klamidospora umumnya tersusun tunggal. Semua isolat menyebabkan insidensi penyakit sebesar 100% pada bibit pisang var. Raja Bulu dengan keparahan gejala layu pada daun antara 58.33% sampai 77.78% dan nekrosis pada bonggolnya antara 50.00% sampai 69.44%.
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