12 13Selenium is recognized as an essential micronutrient for humans and animals. One of the main 14 sources of selenocompounds in the human diet is vegetables. Therefore, this study deals with the 15 Se species present in different edible sprouts grown in Se-enriched media. We grew alfalfa, lentil 16 and soy in a hydroponic system amended with soluble salts, containing the same proportion of 17 Se, in the form of Se(VI) and Se(IV). Total Se in the sprouts was determined by acidic digestion 18 in a microwave system and by ICP/MS. Se speciation was carried out by enzymatic extraction 19 (Protease XIV) and measured by LC-ICP/MS. The study shows that the Se content of plants 20 depends on the content in the growth culture, and that part of the inorganic Se was 21 biotransformed mainly into SeMet. These results contribute to our understanding of the uptake of 22 inorganic Se and its biotransformation by edible plants. 23 24
The present study reports arsenic analysis in Lentinula edodes, Agaricus bisporus and Pleurotus ostreatus before and after being cooked. Furthermore, arsenic in raw and cooked mushroom was determined in the gastric and gastrointestinal bioaccessible fractions obtained after simulating human digestion by means of an in vitro physiologically based extraction test (PBET). Several certified reference materials (SRM 1568a, SRM 1570a, CRM 7503-a, BC211 and IPE-120) were analysed to evaluate the proposed methods. Total arsenic content was 1393, 181 and 335μgAskg(-1) for L. edodes, A. bisporus and P. ostreatus, respectively, and decreased by between 53% and 71% in boiled mushroom and less than 11% in griddled mushroom. High bioaccessibility was observed in raw, boiled and griddled mushroom, ranging from 74% to 89% and from 80% to 100% for gastric and gastrointestinal extracts, respectively, suggesting the need to consider the potential health risk of consumption of the mushrooms analysed.
Research HighlightsSelenium species present in edible plants, grown in Se-enriched peat, are studied.Selcote Ultra® and selenium sodium salts are assayed to increase uptake of Se.Fortification with Se sodium salts increases Se content in plants.Plants biotransform inorganic Se mainly to SeMet.Contents over 10 mg Se kg -1 in peat can damage or inhibit plant growth.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.