The study describes a theoretical model of flow pattern of a newtonian liquid in a flat-bottomed cylindrical vessel with radial baffles at wall agitated by a standard six-blade disc turbine impeller, under turbulent flow. The model, comprising the description of the field of main mean velocity components and of Stokes stream function in the system, is identified by experimental determination of velocity field by means of Pilot direction tubes and hot-film anemometer. The resulting analytical description of the flow pattern in the vessel corresponds well to that found experimentally. Knowing three parameters derived from the velocity profile of the stream streaking from the impeller, the description depends only on the system geometry.

An immortal cell line, MMSV-1, has been developed which exhibits many features of the common luminal epithelial cell of the human mammary gland. The cell line was developed by microinjection of SV40 DNA into individual cells in selected colonies in cultures of human milk epithelial cells. Immunohistochemical staining shows that the MMSV-1 cells express keratins 7, 8, 18 and 19 homogeneously in organized filaments which lead into well-developed desmosomes. They do not express vimentin or keratins found in stratified epithelia or keratin 14 found in basal cells in the mammary gland. The PEM mucin, recognized by the antibody HMFG-I, is also expressed and appears to be processed normally. Fibronectin is detected but shows the punctate pattern typical of cultured normal milk epithelial cells. MMSV-1 cells show a reduced requirement for added growth factors, including cyclic AMP-elevating agents, but do not grow in agar or form tumours in nude mice. Since the transfected cells could be selected on the basis of an extended in vitro life span, antibiotic resistance markers were not introduced and the cells remain sensitive to hygromycin and neomycin.

The presented work is aimed at the development of nontoxic nanocrystalline silicon fluorescence labels, biodegradable in living body and long-term stable, and of fluorescent nanodiamonds mainly for in vitro use. These novel fluorescence labels could be very good substitutes for commercially used quantum dots (e.g. cadmium compound quantum dots) which can be toxic according to the latest results. In this work, manufacturing of porous nanocrystalline silicon (por-Si) is described, several basic optical properties of por-Si are presented and the influence of Si nanocrystals, nanodiamonds, and milled silicon on the growth of a cell culture of L929 mouse fibroblast and HeLa cells is compared. Bio-interaction of nanoparticles was studied by optical transmission microscopy, time-lapse microphotography of cell culture evolution, fluorescence microscopy, fluorescence microspectroscopy, and scanning electron microscopy. The size and shape of nanocrystals were determined using atomic force microscopy (AFM).

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