Karate is a form of martial art that rely on strength, speed, agility, accuracy and breathing so that the athletes are required to have a good lung and heart endurance to have an optimum performance in training/competing. Ambon banana is one of the energy source used to increase endurance for the athletes because it provides carbohydrate and potassium which help the energy metabolism. The purpose of this study was to determine the effect of Ambon banana on the lung and heart endurance of the Karate athletes at STKIP Pasundan Cimahi. The research design used in this study is  pre-experimental design (pre and post test one group) with the sampling design using purposive sampling. Two hours before the Balke testing, a total of 18 subjects were given 150 g of banana Ambon. The result of this study showed that there was an effect of banana Ambon on lung and heart endurance (p<0.05). There is a need for a nutritionist to provide informations regarding feeding regimen for athletes who are in accordance with their needs to achieve maximum endurance. Key words: Karate, cardiorespiratory endurance, VO2max, Ambon banana.