Purpose. To improve a method of organizational culture assessment for making management decisions in cooperation of business and education. Methodology. The study is based on the data of the leading enterprises producing plastics in Ukraine over the period of 20152019. Through the method of binary logic for the purposes of assessing the proposed components of organizational culture of enterprises (intellectual, technical-technological, social, legal and regulatory, professional, communicative, informational), the levels of this culture in plastics manufacturers are determined in mathematical terms. Findings. Based on the generalization of the results of theoretical research on the economic essence of the organizational culture of the enterprise, its key components and the main parameters characterizing them are identified. Public JSC Ukrplastic is identified as the leading company producing plastic products in Ukraine with the highest performance in organizational culture by individual components of the enterprise. The other analyzed companies have medium level of organizational culture indicators. Originality. The research is conducive to the studies on organizational culture, especially the perspective of its usage as a tool of cooperation between Ukrainian plastic producers as the business representatives and educational sector. Practical value. The results of the study can be used by practitioners, scientists, government officials (Ministry of Education and Science of Ukraine and its structural departments, local governments in the fields of education and science) to monitor the development of the information economy.