Myelin oligodendrocyte glycoprotein (MOG) is a protein widely used in the
induction of experimental autoimmune encephalomyelitis (EAE) for studying
human multiple sclerosis (MS). In C57BL/6 female mice aged eight weeks, we
administered subcutaneously MOG35-55 peptide in CFA (complete Freund?s
adjuvant) along with pertussis vaccine injected intraperitoneally. We
observed the sign of flaccid tail as early as thirteen days post-immunization
in five of twelve animals. Hematoxylin and eosin staining of
paraffin-embedded sections of lymph nodes and spleen revealed the presence of
germinal centers in the immunized animals. In the control group of animals,
lymphoid follicles without germinal centers were observed.
Immunohistochemical staining of spleen sections revealed an expression of MHC
II molecules in the EAE-induced group. We would like to point out that even
though the clinical signs are mild, the morphological changes are apparent in
the lymph nodes and spleen of MOG35-55-immunized mice.
Literature data suggest possible link between influenza vaccination and development of autoimmune processes. Therefore, the aim of the study was to investigate the effect of influenza vaccination on spatial learning in mice with experimental autoimmune encephalomyelitis (EAE). EAE was induced in eight-week-old C57BL/6J female mice by subcutaneous immunization (MOG35–55 in complete Freund’s adjuvant) and Pertussis vaccine injected intraperitoneally. Mice were vaccinated with influenza vaccine three days before MOG immunization. The hippocampal-dependent spatial learning test, Morris Water Maze test (MWM), was performed before and after EAE induction. Significant difference (P < 0.05) in the time for completing the Morris Water Maze task was found between mice with mild clinical signs of EAE when compared to other mice. However no significant difference was observed between mice with EAE and mice with EAE that were vaccinated with influenza vaccine. Hippocampal tissue lesions in EAE mice are in correlation with memory impairment. Study shows no influence of influenza vaccine on progression of clinical signs of EAE, spatial learning and memory impairment.
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