Samples of Coffea arabica were collected during the different stages of the fermentation from two production sites in Tanzania

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The effects of volatile compounds produced during coffee processing by Pichia anomala, P. kluyveri and Hanseniaspora uvarum on growth of Aspergillus ochraceus and production of ochratoxin A (OTA) were studied. On malt extract agar (MEA) and on coffee agar (CA), exposure of A. ochraceus to the gaseous phase of malt yeast glucose peptone (MYGP) plates inoculated with P. anomala, P. kluyveri and H. uvarum inhibited fungal growth, with the two Pichia spp. showing the strongest effect. The main esters and alcohols produced by the three yeasts were ethyl acetate, isobutyl acetate, 2-phenyl ethyl acetate, ethyl propionate and isoamyl alcohol. The individual esters and alcohols were found to affect fungal growth. The most effective compound in inhibiting fungal growth was 2-phenyl ethyl acetate; which at 48 µg/l headspace completely inhibited growth of A. ochraceus. Exposure of A. ochraceus to the gaseous phase of MYGP plates inoculated with P. anomala, P. kluyveri and H. uvarum prevented production of OTA. On CA medium, only the headspace of P. anomala and P. kluyveri prevented OTA production. Furthermore, when A. ochraceus was exposed to the headspace of the individual volatile compounds, 2-phenyl ethyl acetate was the most effective in preventing OTA production. Prevention of OTA seems to be due to reduction of fungal biomass.

The effects of Pichia anomala, Pichia kluyveri and Hanseniaspora uvarum predominant during coffee processing on growth of Aspergillus ochraceus and production of ochratoxin A (OTA) on malt extract agar (MEA) and on coffee agar (CA) were studied. The three yeasts were able to inhibit growth of A. ochraceus when co-cultured in MEA and CA. Growth inhibition was significantly higher on MEA than on CA. Furthermore, P. anomala and P. kluyveri were found to have a stronger effect on growth of A. ochraceus than H. uvarum. The three yeasts were able to prevent spore germination of A. ochraceus in yeast glucose peptone (MYGP) broth. In yeast-free supernatant of MYGP broth after an incubation period of 72 h, spores of A. ochraceus were able to germinate with very short germ tubes, but further development of the germ tubes was inhibited. The three yeasts decreased the pH of MYGP broth from 5.6 to a range of 4.4-4.7, which was found to have no effect on spore germination of A. ochraceus. P. anomala, P. kluyveri and H. uvarum were able to prevent production of OTA by A. ochraceus when co-cultured on MEA. On CA medium, P. anomala and P. kluyveri prevented A. ochraceus from producing OTA. H. uvarum did not affect production of OTA by A. ochraceus on CA medium.