Beetroot (Beta vulgaris L.) is one of the plants that contain biologically active compounds that have a function in the prevention and treatment of a wide variety of diseases. The study aims to design new cookies that will support certain groups, such as schoolchildren who may be anaemic. Also, to determine four cookie treatments that were planned to substitute white wheat flour with extraction rate of 72% as follows: T₀ (0%), T₁ (2.5%), T₂ (5.0%), T₃ (7.5%), and T₄ (10%) of beetroot powder to replace 100 g of flour; the cookies were baked at 180 °C for 30–35 min. The chemical composition was assessed, included total phenols, flavonoids, and minerals. Furthermore, during a three-week storage period, antioxidant activity and betalain pigments were evaluated, and sensory evaluation and microbiological assessment were done. Sensory evaluation revealed that the replacement ratio of 10% beetroot was acceptable to the cookies manufactured from white wheat flour with extraction rate of 72%. Compared to the control, a slight decrease was found in the total count of bacteria, fungi, and moulds. We recommend baking beetroot-enriched cookies since it enhances the organoleptic and microbiological characteristics.

The objective of this research was to study the feasibility of using roselle calyces and cinnamon extracts as enriching bioactive components in ice milk making. Formulations of ice milk containing 2%,4% roselle extract and 10%,15% cinnamon extract was carried out. The chemical analysis of roselle extract indicated higher total phenolic, total flavonoids, tannic acid, total antioxidant and anthocyanin contents, compared with cinnamon extract. The highest overrun were observed in the control sample and ice milk with roselle calyces extract of 2%. The control samples had the lowest flavonoid, anthocyanin and tannic acid contents, while ice milk samples with roselle extracts 2 and 4% additive ratio were of the greatest concentration of flavonoid, anthocyanin and tannic acid. Phenolic content of most ice milk samples showed slight increases during the storage at -18 o C for 30 days. The total antioxidant content (TAC) increased in the ice milk by increasing the ratio of roselle calyces extracts and cinnamon extract. The differences in sensory scores of ice milk samples were no statistically significant (P>0.05) in the colour, flavour , taste, mouth feel and texture. The total bacterial count of the ice milk formulations was within the safe levels.

By increasing the undesirable side effects of synthetic food pigments on human health, using safe natural food pigment become an urgent issue. Incorporate corn starch with oils conducted a high impact on red pigment production by Monascus purpureus. Fortification the medium with sesame oil raised the pigment production by 80% and the dry mass by 63% compared with free oil medium. Response surface methodology maximizes the production with 114.6% (12.8 A 500) using medium constituents (g/l); Sesame oil 5; Corn starch 30; Yeast extract 1.5; KH 2 PO 4 2.5 and MgSO 4 .7H 2 O 0.1. After evaluating red pigment stability in three common food components, citric acid showed a great effect on residual stability percentage compared with ascorbic and salicylic acid which decrease slightly the residual stability percentage at light and dark conditions. The mitotic index of red pigment was lower than the negative control at all tested concentrations. Different types of mitotic chromosomal abnormalities e.g. lagging chromosome, chromosomal bridge, chromosome and chromatin fragments, outside chromosome, chromosomal stickiness and micro nuclei were recorded. Insignificant increase in total mitotic aberrations percentage in all tested root tips treated with all concentrations of red pigment (1.23, 1.58, 1.63, 2.32 and 2.40%) compared with negative control (0.91%). There was a significant increase in total aberrations percentage after treatment with all concentrations (10, 15, 20 and 25%) of positive control (2.93, 3.00, 3.55 and 6.53 respectively) except (5%) which was insignificant (2.71%). From the previous data, this red pigment can be used as an alternative safe pigment in the food industry. Keywords Food Á RSM Á Citric acid Á Mitotic index Á Chromosomal aberrations Abbreviations RSM Response surface methodology MIRCEN Microbiological resources center PDA Potato dextrose agar medium YPD Yeast peptone dextrose medium CCD Central composite design ANOVA Analysis of variance DW Distilled water MI Mitotic index Electronic supplementary material The online version of this article (