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Phosphorus deficiency (P2O5) is among the main abiotic stresses that limit soybean production in cerrado soils. Its low availability in soils, especially in the more weathered and acidic ones, the increased demand and its scarcity estimations leadto the need for the development of cultivars more efficient in the use of this mineral. This experiment was conducted under field conditions in the municipality of Imperatriz, state of Maranhão, Brazil, under planosol conditions, in a randomized complete block design, with treatments arranged in a 2x4 factorial scheme with four replicates, four P levels (0, 60, 120 and 180 kg ha-1), and two soy bean cultivars (BRS 333 RR and BRS 9180 IPRO) indicated for tropical regions or Brazilian cerrado. In this study, P2O5 efficiency use was evaluated based on plant height, shoot dry mass (SDM), pod dry mass (PDM) and number of pods (NP). BRS 333 RR cultivar obtained the highest efficiency with doses of 84.35 kg ha-1 and 94.46 kg.ha-1, producing 696,76.76g e 86,72.06g for SDM and PDM respectively, average height of94.15 cm with 123.98 kh.ha-1 and phosphate fertilizer use efficiency (FUE) of 13%
Miniature rose (Rosa chinensis Jacq.) is a shrub-plant belonging to Rosaceae family, originated from Asia and cultivated in Brazil for medicinal and ornamental uses. In August 2016, symptoms and signs of powdery mildew were observed on leaves of miniature rose in Guaramiranga municipality, Ceará State. A specimen (OID99) was collected from symptomatic leaves and examined in the Plant Pathology Laboratory of Embrapa Agroindústria Tropical, Fortaleza, Ceará. In the morphological analysis, the fungus presented epiphytic mycelia and erect, septate, hyaline and unbranched conidiophores. The basal cells were cylindrical, straight and slightly curved at the base. Conidia (n = 50) formed chains at the conidiophore apex and were unicellular, hyaline, ellipsoidovoid to doliiform, with fibrosin bodies, measuring 22.7 to 31.9 μm long and 12.4 to 19.5 μm wide. Appressoria in mycelia were simple and nipple-shaped. Chasmothecia were not observed. The morphological characteristics observed were typical of the asexual stage of Podosphaera sensu Braun and Cook (2012). The genomic DNA was extracted with DNeasy Plant Mini Kit (QIAGEN) from pathogen mycelia collected on the host leaves. The polymerase chain reaction (PCR) amplifications of the internal transcribed spacer (ITS) from the rDNA were conducted with ITS1F / P3R primers (Meeboon and Takamatsu 2015). The PCR product Compliance with ethical standards This article does not contain any studies with human participants or animals performed by any of the authors. Conflict of interest The authors declare that have no conflict of interest.
RESUMO Os fungos pertencentes à família Botryosphaeriaceae são potenciais fitopatógenos e por apresentarem hábito cosmopolita, parasítico, endófitico e uma grande diversidade genética, morfológica e ecológica têm uma considerável importância nos estudos de doenças de plantas. A dificuldade para esporulação e os longos períodos de tal etapa no ciclo reprodutivo dos fungos desta familia são as principais limitações para a manipulação dos mesmos in vitro. Diante disto, este trabalho objetivou testar frutos de ata (Annona squamosa) como meio de cultura alternativo, afim de obter tempos de esporulação inferiores aos já descritos na literatura. Por fim, a utilização dos frutos de ata como meio de cultura demonstrou-se eficiente na esporulação dos isolados testados. Alem disso, dois dos isolados avaliados so foram capazes de esporular nos frutos de ata.
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