Tree age affects wood formation and yield. However, the underlying mechanisms are poorly understood, particularly at the molecular level. In this study, we investigated the transcriptomic changes of the uppermost main stems of Larix kaempferi in an entire rotation period using the RNA-Seq method. In total, ∼151 million reads were obtained from the stems of 1-, 2-, 5-, 10-, 25-, and 50-year-old L. kaempferi trees. Combining these with the published Illumina sequencing reads, 299,637 assembled transcripts were generated, of which 161,232 were annotated. Time series expression profiling identified 12,927 transcripts as differentially expressed genes (DEGs); function enrichment analysis of these DEGs showed that 459 gene ontology terms in the biological process category were enriched. These terms were associated with the processes of wood formation, such as cell differentiation, growth and death, and its hormonal regulation. Based on the expression patterns of L. kaempferi homologues of genes associated with ethylene, calcium, and cell wall expansion and synthesis, the regulatory network of tracheid growth was outlined. Altogether, the comparative transcriptomic analysis reported here demonstrated the molecular aspects of aging effects on L. kaempferi wood formation. The identification of genes involved in the regulatory network of tracheid growth provides a means of investigating the regulation of wood formation in gymnosperm trees and also offers potential targets for genetic manipulation to improve the properties of xylem fibers.
Abstract. Objective To explore the reference value of molecular immunological detection in the clinical diagnosis and treatment of type I and II schizophrenics. Methods 86 type I and II schizophrenics admitted and treated in our hospital from February 2015 to January 2017, together with 45 healthy subjects receiving molecular immunological detection at the same period were treated as the objects of study. They were divided into group I (n=41), group II (n=45) and control group (n=45). The molecular immunological detection results in each group of subjects were compared. Results Compared with control group, IgG and C4 levels in group I were remarkably higher, while IgM level was notably lower; meanwhile, IgA, IgM, IgG and C4 levels in group II were evidently higher. Compared with group I, IgM level in group II was distinctly higher, with the difference being statistically significant (P<0.05). The prognostic recovery effect in group I was superior to that in group II after treatment (P<0.05). Conclusion Molecular immunological detection results have certain reference value to the clinical diagnosis and treatment for type I and II schizophrenics.
Abstract. To explore the application effect of live attenuated vaccine in preventing mumps. 224 children vaccinated with live attenuated vaccine in our hospital from April 2015 to 2016 were selected as the objects of study. Live attenuated vaccine was applied in immune prevention, and the immunological effect and protective effect were evaluated and compared with 200 children that had not been vaccinated. The positive immune detection rate in vaccinated patients was relatively higher than that before (95.54%>66.51%). The immune success rate was 64.73%; of them, immune success rate in patients with the mumps antibody titer measured value of 1:2-1:6 was as high as 100%. The mumps infection rate (0.89%<7.5%) and complication incidence (0<1.5%) in vaccinated children were remarkably lower than those in non-vaccinated inspected children, with the differences being of statistical significance (P<0.05). The live attenuated vaccine shows outstanding superiority during mumps prevention. The risk of disease incidence can be effectively reduced after taking full advantage of its immune protection.
Abstract. This paper aimed to introduce cytochrome P450 (CYP450) enzymes and illustrate the enzyme catalytic mechanisms. Notably, this paper had focused on the relation of CYP450 with microbial drug preparation; meanwhile, it had pointed out the challenges faced by the future development of P450 enzymes.
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